响应面法优化香椿中γ-谷氨酰转肽酶的超声波辅助提取条件

Optimization of ultrasonic- assisted extraction conditions ofγ-glutamyltranspeptidase from Toona sinensis by response surface methodology

采用响应面分析方法对香椿中的γ-谷氨酰转肽酶(GGT)超声波辅助提取的工艺进行优化。以单因素实验为基础,以中心组合实验设计为原理,采用三因素三水平的响应面分析方法,将GGT的提取量作为响应值来进行回归分析。得到超声辅助提取GGT的最佳条件:提取功率260 W、提取时间28 min、缓冲液体积240 m L(样品质量10.00 g)。在上述提取条件下,提取液中GGT的含量达到7.188 U/g,与模型预测值基本相符,说明该优化方法合理可行。

In order to optimize the ultrasonic-assisted extraction of -y-glutamyltranspeptidase （GGT）from Toona sinensis, single factor investigations were initially carried out, followed by construction of a three-factor,three-level Box-Benhnken experimental design, quadratic regression fitting for GGT activity as a function of three extraction conditions and response surface analysis.The optimal conditions for the ultrasonic-assisted extraction of GGT were volume of buffer solution of 240 mL（10.00 g） ,ultrasonic power of 260 W,and ultrasonic time of 28 min. Under the above conditions,the experimental value of GGT activity was 7.188 U/g,which was in basic agreement with the model predicted value.