摘要
目的了解临床分离的非O1/O139群霍乱弧菌毒力及耐药特征。方法收集2014 2015年北京友谊医院410月肠道门诊分离到的非O1/O139群霍乱弧菌,采用微量肉汤稀释法检测霍乱弧菌对15种抗生素的耐药性;PCR检测霍乱弧菌的毒力相关基因。结果 35株非O1/O139群霍乱弧菌对复方新诺明的耐药率（40.0%）最高,其次是氯霉素（28.5%）和磺胺异恶唑（22.6%）,对阿莫西林/克拉维酸、头孢曲松、头孢西丁、头孢吡肟及亚胺培南完全敏感。毒力基因检测显示所有菌株均携带hly A和omp U,hap A（97.1%）、rtx A（91.4%）、Ⅵ型分泌系统T6SS（94.3%~97.1%）、Ⅲ型分泌系统T3SS（80.0%~85.7%）和nan H（62.9%）阳性率较高;主要的毒力基因型为hly A-rtx A-hap A-omp U-nan H-vas A-vas Kvas H-vcs C-vcs V-vcs N-vsp D（40.0%）。结论临床分离非O1/O139群霍乱弧菌毒力基因多样化,对抗菌药物耐药性较高,需加强腹泻病例中非O1/O139群霍乱弧菌的毒力及耐药监测。
Objective To explore the distribution of virulence genes and drug resistance of non-O1 / O139 Vibrio（ V.）cholerae. Methods Non-O1 / O139 V. cholerae strains and the related patient records were collected from the intestinal clinic of Beijing Friendship Hospital from 2014 to 2015. The susceptibility of V. cholerae strain to 15 antibiotics was tested with microbroth dilution method,and virulence genes were detected by using polymerase chain reaction（ PCR）. Results The drug susceptibility test showed that the resistant rate of 35 strains of non-O1 / O139 V. cholerae to trimethoprim-sulfamethoxazole was highest（ 40. 0%）,followed by the resistant rates to chloramphenicol（ 28. 5%） and sulfisoxazole（ 22. 6%）. The strains were sensitive to amoxicillin/clavulanate,ceftriaxone,cefoxitin,cefepime and imipenem. PCR detection of virulence genes showed that all strains were positive for hly A and omp U gene,and the positive rates of hap A,rtx A,T6 SS,T3SS and nan H were 97. 1%,91. 4%,94. 3%- 97. 1%,80. 0%- 85. 7%,62. 9%,respectively. The predominant virulence pattern was hly A-rtx A-hap A-omp U-nan H-vas A-vas K-vas H-vcs C-vcs V-vcs N-vsp D（ 40. 0%）. Conclusion The high antibiotic resistance and virulence gene polymorphism suggested that it is important to strengthen the surveillance for the genetic variation and drug resistance of the clinical isolates of non-O1 / O139 V. cholerae.
出处
《疾病监测》
CAS
2016年第6期517-521,共5页
Disease Surveillance
基金
北京市自然科学基金(No.7164249)
北京友谊医院科研启动基金资助项目(No.yyqdkt2015-23)~~
