白英总生物碱的抗炎活性研究

Anti-inflammatory Activity of Total Alkaloids from Solanum Lyratum

目的:观察白英总生物碱的抗炎活性。方法:培养人脐静脉内皮细胞(HUVEC),用200μmol·L^(-1)双氧水(H_2O_2)损伤细胞;培养小鼠单核/巨噬细胞(RAW 264.7),用脂多糖(LPS)诱导;抽签法随机分成正常组、模型组、阳性对照组、白英总生物碱低、中、高剂量组。给药后,继续培养,应用CCK-8法观察细胞存活率;饲养SD大鼠,抽签法随机分为正常组、模型组、阳性对照组、白英总生物碱低、中、高剂量组,观察白英总生物碱对角叉菜胶诱导大鼠急性踝关节肿胀度的影响,测定前列腺素E2(PGE_2)、环氧化物水解酶-2(COX-2)含量。结果:白英总生物碱中剂量对H_2O_2损伤的HUVEC细胞的影响以及高剂量组对LPS诱导的巨噬细胞RAW 264.7的影响,均和阳性对照组相当,差异无统计学意义(P>0.05);中、高浓度白英总生物碱能够明显降低大鼠急性踝关节肿胀度,降低大鼠足趾渗出液中PGE2、血清中COX-2含量,与模型组差异有统计学意义(P<0.01)。结论:白英总生物碱具有明显的抗炎活性,有必要进一步深入研究其药效及作用机制。

Objective：To study the anti-inflammatory activity of total alkaloids from Solanum lyratum.Methods：Human umbili-cal vein endothelial cells （ HUVECs） were cultured and induced by H 2 O2 （200 μmol＆#183; L-1 ） , and RAW 264.7 cells were cultured and induced by lipopolysaccharide （ LPS ） .The two inflammatory cell models were randomly divided into the normal group , model group, positive control group, and total alkaloids group respectively at low , medium and high dose.After the treatment, the cells were continued to be cultured , and CCK-8 method was applied to observe the cell survival rate .SD rats were randomly divided into the nor-mal group, model group, positive control group, total alkaloid group respectively at low, medium and high dose,and then the rats re-ceived subplantar injection of carrageenan in the paw .After the treatment , inflammation was analyzed by the swelling degree of acute ankle joint injury, and the contents of prostaglandin E2 （PGE2） and cyclooxygenase-2 （cox2-） were detected.Results：The effect of total alkaloids from Solanum lyratum at medium dose on H 2O 2-induced HUVECs and that of total alkaloids from Sola num lyratum at high dose on LPS-stimulated macrophages were similar to that of the positive control group without statistical significance （P〉0.05）, and the total alkaloids from Solanum lyratum at medium and high dose could significantly reduce the swelling degree of the acute ankle model in rats （P〈0.01）, and decrease the content of PGE2 in the toe exudate of rats and that of COX-2 in serum （P〈0.01） with statistical significance when compared with that in the model group （P〈0.01）.Conclusion：The total alkaloids from Solanum lyra-tum have significant anti-inflammatory activity , and it is necessary to further study the efficacy and action mechanisms .