阿托品光学异构体的毛细管电泳法拆分研究

Study on Enantiomer Separation for Atropine by Capillary Electrophoresis

目的:考察阿托品光学异构体的毛细管电泳拆分方法。方法:采用毛细管电泳法,弹性石英毛细管柱(60cm×75"m,有效长度40cm),磷酸盐缓冲液浓度:30mmol·L^(-1),重力进样高度差10cm,进样时间:5s,检测波长:225nm。分别对手性拆分剂种类及浓度、缓冲溶液的pH、运行电压、有机溶剂等进行考察,优选出最佳拆分条件。结果:阿托品光学异构体最佳拆分条件为:缓冲液pH为7.0,磺酸化-β-环糊精聚合物(S-β-CDP)浓度为10mg·ml^(-1),电压为12kV。结论:本方法简便、快速,能实现对阿托品光学异构体的拆分。

Objective：To investigate the enantiomer separation for atropine by capillary electrophoresis .Methods：Capillary elec-trophoresis was used with an elastic quartz capillary column （60 cm ×75 mm, effective length of 40 cm）.The concentration of phos-phate buffer was 30 mmol· L-1 .The high and time of injection was 10 cm and 5 s, respectively.The detection wavelength was 225 nm.The best separation conditions were selected including the type and concentration of chiral resolving agent , pH of the buffer solu-tion, operating voltage and organic solvent.Results：The optimum conditions of separation were as follows：the pH of buffer solution was 7.0, the concentration of S-β-CDP was 10 mg· ml-1 , and the operating voltage was 12 kV.Conclusion： The method is simple and fast, which can be used to se parate the optical isomers of atrpo ine.