HPLC法同时测定田基黄颗粒中3种黄酮类成分的含量

Simultaneous Determination of Three Flavonoids in Tianjihuang Granules by HPLC

目的：建立测定田基黄颗粒中3种黄酮类成分含量的高效液相色谱方法。方法：用ZORBAX Eclipse XDB-C18色谱柱,流动相为甲醇-0.2%磷酸溶液（42∶58）,检测波长为350 nm,流速1.0 ml·min-1。结果：异槲皮苷、槲皮苷、槲皮素分别在4.572~114.300μg·ml-1、7.028~175.700μg·ml-1、2.195~54.880μg·ml-1浓度范围内呈良好线性关系（r分别为0.999 8、0.999 8、0.999 9）;平均加样回收率分别为98.01%（RSD=1.24%,n=6）、98.14%（RSD=0.98%,n=6）、97.00%（RSD=1.09%,n=6）。结论：该方法专属性强、操作简便,可以用于田基黄颗粒的质量控制。

Objective： To establish an HPLC method for the simultaneous determination of three flavonoids in Tianjihuang gran -ules.Methods：A ZORBAX Eclipse XDB-C18 column was used.The mobile phase was methanol-0.2%phosphoric acid solution （42∶58）.The detection wavelength was 350 nm and the flow rate was 1.0 ml· min-1.Results：The linear range of isoquercitrin, quer-citrin and quercetin was 4.572-114.300 μg· ml-1（r=0.999 8）, 7.028-175.700 μg· ml-1（r=0.999 8） and 2.195-54.880 μg· ml-1（r=0.999 9）, respectively.The average recovery was 98.01%（RSD=1.24%, n=6）, 98.14%（RSD=0.98%, n=6） and 97.00%（RSD=1.09%, n=6） , respectively.Conclusion：The method is specific and simple,which can be used for the quality con-trol of Tianjihuang granules.