多探针FISH技术在检测喀什地区维吾尔族急性白血病患者细胞遗传学异常中的应用

Nursing experience of the multi-probe FISH technology applied in the detection of abnormal cell genetics of patients with acute leukemia of Uygur ethnic group in Kashgar Uygur zone

目的：探讨多探针FISH技术在检测喀什地区维吾尔族急性白血病患者细胞遗传学异常中的应用效果。方法临床纳入新疆喀什地区第一人民医院和广东省第二人民医院2013年1月至2015年12月期间收治的100例急性白血病细胞遗传学异常患者,收集患者的骨髓标本,均行预包被式多探针FISH技术与常规染色体核型分析技术检测,并进行对比分析；同时,对患者实施护理干预措施。结果100例急性白血病患者中,50例急性髓系白血病（ AML）患者多探针FISH技术检测率60.0%明显高于常规染色体异常核型检测率28.0%,50例急性淋巴细胞白血病（ ALL ）患者多探针 FISH 技术检测率58.0%明显高于常规染色体异常核型检测率26.0%,差异均有统计学意义（ P〈0.05）。结论喀什地区维吾尔族急性白血病患者细胞遗传学异常采用多探针FISH技术检测,具有省时、高效且准确率高等优点,值得临床上作为诊断急性白血病的重要手段推广应用。

Objective To explore the nursing experience of the multi-probe FISH technology applied in detec-tion of abnormal cell genetics of patients with acute leukemia of Uygur ethnic group in Kashgar Uygur zone . Methods A total of 100 cases of patients with acute leukemia cytogenetic abnormalities who were treated in the First People＇s Hos-pital of Kashgar and the Second People＇s Hospital of Guangdong Province from January 2013 to December 2015 were se-lected as the research objects. The patients＇ bone marrow specimens were collected. And all the patients were treated with pre-coating multi-probe FISH technique and conventional chromosome karyotypes analysis detection technology, and the data was analyzed with the method of contrast analysis;at the same time, nursing intervention measures were imple-mented to the patients. Results Among the 100 cases of acute leukemia patients, 50 cases of acute myeloid leukemia （ AML） patients were detected with multi-probe fish detection, and the detection rate was 60. 0%, which was signifi-cantly higher than that of the conventional chromosome abnormal karyotype detection rate of 28. 0%, 50 cases of acute lymphoblastic leukemia （ ALL ） patients were detected by multi-probe FISH technique and the detection rate was 58. 0%, which was significantly higher than that of the conventional chromosomal abnormal karyotype detection rate of 26. 0%, and there were statistically significance （ P〈0. 05 ） . Conclusions The multi-probe FISH technology was ap-plied in the detection of abnormal cell genetics of patients with acute leukemia of Uygur ethnic group in Kashgar Uygur zone, which shared the advantages of time saving, high efficiency and high accuracy, and it is worth being used as the clinical diagnosis method for acute leukemia and meanwhile it is worth a popularization and application.