Effect of acupuncture combined with medicated thread moxibustion of Zhuang medicine on insomnia rats injected with orexin in lateral ventricle

针刺联合壮医药线点灸对侧脑室注射orexin失眠大鼠的影响（英文）

Objective To explore the effect of acupuncture combined with medicated thread moxibustion of Zhuang medicine on insomnia rats. Methods Sixty SPF Wistar rats were randomly divided into four groups, including a normal control group （control group）, a sham operation group, a model group and a treatment group, with 15 rats in each group. In the model group and treatment group, insomnia model were established by injecting orexin into lateral ventricle of rats. In the sham operation group, artificial cerebrospinal fluid was injected into lateral ventricle of rats, and the rats in control group were not treated. On the basis of modeling, the rats in treatment group were treated by acupuncture combined with medicated thread moxibustion of Zhuang medicine which was conducted once a day, and the treatment lasted for 14 days. The expression of orexin mRNA and orexin content were detected, and the changes of associated receptors, including orexin 1 receptor （OX I R） and orexin 2 receptor （OX 2 R） were observed. Results The expression of orexin mRNA in model group （6.54 ± 1.21） significantly increased when compared with control group （0.66±0.12, P〈0.01）, and orexin content in model group [（i 156.35±126.88） pg/mL] significantly increased when compared with control group [（427.12_± 32.54） pg/mL, P〈O.OI]. The expression of orexin mRNA in treatment group （0.87 ± 0.26） significantly reduced when compared with the model group （P〈0.01）, and orexin content in the treatment group [（585.23 ± 89.21） pg/mL] significantly reduced when compared with the model group （P〈O.01）. The percentage of OX I R positive cells in the model group （0.64±0.15） significantly increased when compared with the control group （0.21±0.03, P〈0.01）, and the percentage of OX 2 R positive cells in the model group （0.76±0.18） significantly increased when compared with the control group （0.24±0.01, P〈O.01）. The percentage of OX 1 R positive cells in the treatment group （0.36_±0.08） significantly reduced when compared with the model group （P〈0.01）. The percentage of OX 2 R positive cells in treatment group （0.25_± 0.03） significantly reduced when compared with model group （P〈0.01）. Conclusion It might be an effective link of acupuncture combined with medicated thread moxibustion of Zhuang medicine in treatment of insomnia that the treatment could inhibit the awakening promotion effect of orexin by affecting orexin, OX I R and OX 2 R.

目的:探讨针刺联合壮医药线点灸对失眠模型大鼠的影响。方法:SPF级Wistar大鼠60只随机分为正常对照组(简称对照组)、假手术组、模型组、治疗组共4组,每组15只。模型组和治疗组采用侧脑室注射orexin制备失眠大鼠模型。假手术组侧脑室注射人工脑脊液,对照组不予特殊处理。在造模基础上,治疗组采用针刺联合壮医药线点灸疗法进行治疗,每日1次,连续治疗14 d。检测orexin m RNA表达、orexin含量以及观察orexin相关受体OX 1 R(orexin 1 receptor)和OX 2 R(orexin 2 receptor)变化。结果:与对照组orexin m RNA表达(0.66±0.12)比较,模型组orexin m RNA表达(6.54±1.21)显著升高(P<0.01);与对照组orexin含量[(427.12±32.54)pg/m L]比较,模型组orexin含量[(1 156.35±126.88)pg/m L]显著升高(P<0.01)。与模型组orexin m RNA表达比较,治疗组orexin m RNA表达(0.87±0.26)显著下降(P<0.01);与模型组orexin含量比较,治疗组orexin含量[(585.23±89.21)pg/m L]显著下降(P<0.01)。与对照组OX 1 R阳性细胞百分率(0.21±0.03)比较,模型组OX 1 R阳性细胞百分率(0.64±0.15)显著升高(P<0.01);与对照组OX 2 R阳性细胞百分率(0.24±0.01)比较,模型组OX 2 R阳性细胞百分率(0.76±0.18)显著升高(P<0.01)。与模型组比较,治疗组OX 1 R阳性细胞百分率(0.36±0.08)下降明显(P<0.01),OX 2 R阳性细胞百分率(0.25±0.03)也下降明显(P<0.01)。结论:针刺联合壮医药线点灸疗法通过影响orexin和OX 1 R及OX 2 R,抑制orexin促觉醒作用,可能是其治疗失眠的一个起效环节。