Fn14-shRNA慢病毒载体的制备

Construction and identification of Fn14-shRNA lentiviral vector

目的构建Fn14-shRNA慢病毒载体,以进一步进行动物实验,研究Fn14在骨肉瘤中的表达和作用。方法使用RNA干扰技术,构建Fn14干扰载体、病毒包装、感染细胞,并进行鉴定。结果 Fn14-shRNA干扰质粒单酶切验证阳性,测序结果与设计完全一致,干扰组Fn14在稳转细胞株中表达下调。结论成功构建了Fn14-shRNA慢病毒载体,为进一步从分子水平探讨Fn14在骨肉瘤中的生物学作用奠定了基础。

Objective To construct Fn14-shRNA lentiviral vector to further investigate the expression and biological significance of Fn14 in osteosarcoma with animal experiment. Methods Fn14-shRNA vector was constructed by RNA interference technique,and viral packaging,cell co-transfection,and indentification were conducted. Results The single enzyme detection was positive,and the sequencing result was consistent with design. Fn14 was downregulated in stably transfected cell lines. Conclusion Fn14-shRNA lentivirus recombinant vectors are successfully constructed,which lays the foundation for further study of the biological significance of Fn14 in osteosarcoma.