新疆伊犁地区喀什河谷流域密叶杨遗传多样性分析

Genetic Diversity of Populus talassica Along Kashgar River Basin

为研究新疆伊犁地区喀什河谷流域密叶杨遗传多样性及群体遗传结构与空间地理位置上、中、下游变化的关系,选用26对微卫星标记对220个个体的遗传多样性进行了分析,共检测到163个等位基因,平均等位基因数(Na)为6.629;多态性位点百分率(PPL)为100%,期望杂合度(He)在上、中、下游3个群体中都处于较高水平,分别为0.548、0.567和0.591;分子方差分析(AMOVA)的结果表明,7%的遗传分化来自种群间,遗传变异主要集中在群体内不同个体之间;基因分化系数(Gst)为0.038,表明群体分化处于较低水平,而检测到群体间的基因流(Nm)则为12.818,处于较高水平。表明密叶杨种群的遗传多样性在空间地理位置上未产生分化,遗传多样性水平较高。并提出了密叶杨种群保护策略。

In order to understand the relationship between spatial location and populations of Populus talassica occurring in Kashi River Basin,Ili of the Xinjiang,26 pairs of SSR primers were used.A total of 163 alleles were detected from 220 individuals,with an average of 6.629 alleles per locus,polymorphism percentage level was 100%,and a relatively high expected heterozygosities（He）for populations at upper,middle and lower reaches were 0.548,0.567,and 0.591,respectively.Analysis of molecular variance（AMOVA）showed that differentiation among populations accounted for 7% of the total and most variation existed among the individuals within a population.A differentiation coefficient（Gst）of 0.038 and the value of gene flow（Nm）of 12.818 at a upper reach also supported the conclusion that the differentiation among populations was at the middle and lower reaches.It was concluded that differentiation of the biodiversity of P.talassicadidnot occurred with variation of geographic position,high levels of genetic diversity maintained.Protection strategies of P.talassica were put forward.