结核感染T细胞(IGRA)检测在结核病诊断中的应用

Study of Interferon Gamma Release Assay in the Diagnosis of Tuberculosis

目的探讨结核感染T细胞检测(IGRA)在结核病诊断中的应用价值与临床意义。方法对2015-01/12期间232例患者进行TB-IGRA筛查,将筛查结果与涂片抗酸染色,PCR进行比较分析。结果 232例临床标本的检测结果比较,IGRA检测敏感性91.5%,特异性83.9%;TB-DNA检测敏感性70.6%,特异性91.1%;涂片抗酸杆菌染色检测敏感性21.1%,特异性100%。3组差异有统计学意义(P<0.05)。结论 IGRA实验较传统的结核病检测方法有更好的灵敏度、特异度,对于临床诊断结核具有重要意义,但该实验对区分结核活动性与否,对评估抗结核疗效,还需进一步探讨。

Objective To evaluate the value of interferon gamma release assay （IGRA） for the diagnosis of tuberculosis. Methods A total of 232 patients received tuberculosis interferon -gamma release assay （TB -IGRA） from January 2015 to December 2015 were included in this analysis. The result of TB - IGRA, smear acid fast staining （SAFS） and Polymerase Chain Reaction （PCR） were compared. Results The sensitivity of IGRA, SAFS and PCR were 91.5%, 21.1% and 70.6% respectively. The specificity of those three methods were 83.9%, 100% and 91. 1% respectively. Conclusion When compared with conventional method, IGRA has higher sensitivity and specificity. Further study is still needed for the distinction of activity of tuberculosis and the curative effect.