Fas/FasL在原发免疫性血小板减少症发病机制中的作用研究

Study on role of Fas/FasL in pathogenesis of primary immune thrombocytopenia

目的探讨Fas及FasL系统在成年原发免疫性血小板减少症(ITP)发病机制中的作用。方法收集成年初诊ITP患者及健康成年人外周抗凝静脉血。采用流式细胞术检测ITP患者Th/Tc、Th1/Th2、Tc1/Tc2细胞Fas及FasL的表达率、血小板表面Fas及FasL的表达率。结果与健康对照组相比,ITP组Th、Th1、Th2、Tc、Tc1、Tc2细胞表面Fas及FasL的表达率均明显升高(P<0.05);同时ITP组血小板表面Fas的表达率明显升高(P<0.05),而血小板表面FasL的表达无明显变化(P>0.05)。结论 ITP患者T细胞亚群及血小板表面Fas及FasL表达的异常,可能与ITP发病机制密切相关。

Objective To explore the role of Fas and FasL system in the pathogenesis of adult primary immune thrombocytopenic purpura （ITP）. Methods The peripheral anticoagulant venous blood samptes were collected from the patients with newly diagnosed ITP and healthy adults. The expression rates of Fas and FasL in Th/Tc, Th1/Th2, Tc1/Tc2 cells and theirexpression rates at platelet surface were detected by flow cytometry. Results The Fas and FasL expression rates on the surface of Th,Th1,Th2, Tc,Tc1 and Tc2 in the ITP group were increased compared with the healthy control group（P〈0.05）. ,meanwhile the FasL expression on the platelet surface was significantly increased（P〈0.05）, but the expression of FasL on the platelet surface had no obvious change（P〉0.05）. Conclusion The Fas and FasL expression on T cell subsets and platelet surface in ITP patients is abnormal, which may be related with the pathogenesis of ITP.