猪流行性腹泻病毒S基因序列分析

Sequence Analysis of S Gene in Porcine Epidemic Diarrhea Virus

根据猪流行性腹泻病毒S基因序列设计引物,应用RT-PCR方法从广东省某猪场采集的初生仔猪腹泻样品检测猪流行性腹泻病毒,并将其克隆测序分析。结果表明,病料样品中有猪流行性腹泻病毒,S基因与KNU-0801、KNU-0901亲缘关系最近,处于同一个分支;与弱毒疫苗株CV777的S基因亲缘关系较远,处于另外一个分支。

Sequence Analysis of S Gene in Porcine Epidemic Diarrhea Virus LI Min （Foshan Chancheng Agricultural Products Quality and Safety Supervision and Testing Center, Foshan 528000, China） Abstract： Primers of porcine epidemic diarrhea virus were designed based on the S gene sequence, the intestinal specimens of diarrhea piglets from a pig farm in Guangdong province was tested by RT-PCR to detect porcine epidemic diarrhea virus, and was also cloned, sequenced and analyzed. The result showed that porcine epidemic diarrhea virus ex- ists in the specimens, and S gene is the nearest to KNU-0801 and KNU-0901 which belongs to the same branch, while the S gene in the specimen is distant from the S gene from attenuated vaccine strain CV777, which belongs to different branch.