摘要
目的评价线性探针杂交技术(简称HAIN技术)快速检测耐多药肺结核的临床效果。方法对122例涂阳肺结核患者痰标本同时进行HAIN技术和罗氏培养及药敏试验,检测异烟肼、利福平的耐药情况,并对结果进行比较。结果 HAIN技术对肺结核患者耐多药的检测效果和传统罗氏培养法相比无显著差异(P>0.05)。HAIN技术检测异烟肼和利福平的灵敏度分别为73.08%、82.76%,阳性预测值分别为79.17%、80.00%;检测耐多药的灵敏度为57.89%,阳性预测值为64.71%;所有检测结果的特异度和阴性预测值均>90%。结论 HAIN技术是快速诊断耐多药肺结核的好方法,但检测效果存在一定的局限性。
Objective To evaluate the clinical effect of linear probe assay (HAIN )on fast diagnosis of multi-drug resistance tuberculosis.Methods A total of 122 patients with smear-positive lung tuberculosis were given HAIN,Lwenstein-Jensen (L-J)cultivation and drug susceptibility test,and the resistance to isoniazid and rifampicin was determined.Results Compared with traditional L-J cultivation,the effect of HAIN on lung tuberculosis patients with multi-drug resistance was not significant (P 〉0.05).The sensitivity value of HAIN for isonizid and rifampicin was 73.08% and 82.76%,respectively.Moreover,the positive predictive value was 79.17% and 80.00%.The sensitivity and positive predictive value on multi-drug resistance tuberculosis were 57.89% and 64.71%,respective-ly.The specificity and negative predictive value of all results were higher than 90%.Conclusion HAIN is still ef-fective in fast diagnosis of multi-drug resistance tuberculosis,but it shows a few limitations.
出处
《临床肺科杂志》
2016年第8期1500-1501,1504,共3页
Journal of Clinical Pulmonary Medicine
基金
安徽省卫生和计划生育委员会结核病防治应用课题(No15tb003)
关键词
耐多药结核病
结核分枝杆菌
异烟肼
利福平
multi-drug tuberculosis
mycobacterium tuberculosis
isniazid
rifampicin