复治肺结核患者血清IL-17、IFN-γ和IL-4的表达及其临床意义

Detection and significance of serum levels of IL-17,IFN-γ and IL-4 in patients with retreated pulmonary tuberculosis

目的探讨IL-17、IFN-γ及IL-4等细胞因子在复治肺结核治疗过程中的表达情况及其临床意义。方法采用ELISA法分别测定复治肺结核患者治疗前组(20例)、治疗3个月组(16例)、治疗8个月组(14例)的IL-17、IFN-γ和IL-4水平。对照组为18例健康志愿者。统计分析比较各组间细胞因子水平的差异性。结果 IL-17、IL-4水平,治疗前组高于治疗3月组、治疗8个月组及正常对照组(P均小于0.05);治疗3个月组高于治疗8个月组及正常对照组(P均小于0.05);而IFN-γ水平与IL-17、IL-4水平恰恰相反。治疗8个月组与正常对照组三者水平差异均无统计学意义(P均>0.05)。结论随着抗结核疗程的进行,IL-17、IL-4水平逐渐降低,而IFN-γ水平逐渐升高。提示动态监测这些细胞因子表达情况对判断复治肺结核患者的疗效具有重要的临床意义。

Objective To study the detection and significance of serum levels of interleukin-17 （IL-17）, interferon-γ（IFN-γ）and interleukin-4 （IL-4）in patients with retreated pulmonary tuberculosis.Methods The levels of IL-17,IFN-γand IL-4 were determined by enzyme-linked immunosorbent assay （ELISA）in patients with retreated pulmonary tuberculosis before （20 cases）,3 month （16 cases）and 8 months （14 cases）after anti-tuber-culosis treatment,respectively.There were 18 healthy volunteers as the control group.The data were analyzed by t-test and Pearson correlation analysis.Results The levels of IL-17 and IL-4 were the highest and the level of IFN-γwas the lowest before anti-tuberculosis treatment （P 〈0.05）.8 months after anti-tuberculosis treatment,there was no significant difference with the control group （P 〉0.05）.Conclusion With anti-tuberculosis treatment,the ser-um levels of IL-17 and IL-4 decrease significantly and the level of IFN-γincreases significantly,which indicates that continuous monitoring of immunological indicators is helpful to determine the efficacy of anti-tuberculosis treatment.