癫痫清颗粒对Aβ_(25-35)所致阿尔茨海默病小鼠模型学习记忆的影响

Effects of Dianxianqing Granule on the Learning and Memory in Mice Induced by β-amyloid(Aβ_(25-35))

目的:研究癫痫清颗粒对β-淀粉样蛋白(Aβ_(25-35))致阿尔茨海默病模型(AD)小鼠的学习记忆改善作用。方法:60只昆明种鼠随机分为假手术组,模型组,癫痫清颗粒低(3.12 g·kg^(-1))、中(6.24 g·kg^(-1))和高(9.36 g·kg^(-1))剂量组和盐酸多奈哌齐组(1.30 mg·kg^(-1)),海马内注射(Aβ_(25-35)),制作小鼠AD模型。连续灌胃给药15 d。Y迷宫和Morris水迷宫实验检测各组小鼠认知行为改变,HE染色观察其对AD模型小鼠神经元的影响。结果:癫痫清颗粒可显著增加AD模型小鼠的自发交替反应率,缩短逃避潜伏期,减少总路程,延长游泳路程百分比,可改善海马区神经元细胞排列紊乱、水肿等现象,减少神经元死亡。结论:癫痫清颗粒对Aβ_(25-35)致阿尔茨海默病模型小鼠学习记忆能力具有改善作用。

Objective ： To investigate the effects of Dianxianqing Granule on learning and memory in model mice induced by β-amyloid （ A β 25-35 ） - Methods ： Sixty mice were divided into six groups randomly： sham group, model group, Dianxianqing Granule low dose group （ 3.12 g·kg^-1 ）, Dianxianqing Granule medium dose group （ 6.24g·kg^-1 ） and Dianxianqing Granule high dose group （ 9.36 g·kg^-1 ） and donepezil group （ 1.30g·kg^-1 ）. β -Amyloid protein （ A β 25-3s ） was infused into the hippocampal of mice. Drugs were administrated for 15 days. Y maze and Morris water maze was detected to evaluate the cognitive behavior. HE-staining was used to observe the neuron changes caused by Dianxianqing Granule. Results ： Dianxianqing Granule can increase the alternation behavior significantly, decrease swimming escape latency and total distance, prolong the swimming distance percentage; it also can improve the disorder, edema and other symptoms of hippocampus neurons, reduce the death of neurons. Conclusion： Dianxianqing Granule can improve the effects of learning and memory in mice induced by β-amyloid （Aβ25-35）.