五味子治疗维生素A缺乏型干眼症疗效及作用机制

Effect and Mechanism of Schisandra on Treating Vitamin A-deficient Dry Eye Syndrome

目的:观察五味子治疗维生素A缺乏型干眼症的疗效及眼角膜组织中IL-6、TNF-α的表达变化,比较泪腺细胞凋亡的数量,探讨酸味中药缓解干眼症的作用机制。方法:将180只白色来亨鸡随机分为正常组、模型组(维生素A缺乏组)、维生素A对照组、五味子治疗组(高、中、低剂量组)。分别于用药前、用药后2周、用药后4周对各组来亨鸡进行Schirmer I试验(SIT)和泪膜破裂时间(BUT)检查。对角膜做荧光素染色并用免疫组织化学检测白介素-6(IL-6)和肿瘤坏死因子-α(TNF-α),并比较各组泪腺细胞凋亡的数量。结果:用药2周、4周后,与模型组比较,Schirmer试验滤纸湿长有明显改变,泪膜破裂时间逐渐增加,角膜表面湿润度增多,荧光素染色少量着色。免疫组织化学检测发现IL-6和TNF-α在各组来亨鸡角膜上皮细胞中表达显著降低,泪腺细胞凋亡明显缓解。结论:五味子可以提高泪液基础分泌量,维持泪膜稳定性,其机制可能是抑制泪腺细胞凋亡,抑制IL-6和TNF-α表达,减轻炎症反应。

Objective： Investigate the efficacy of Chinese medicine Schisandra to vitamin A-deficient dry eye syndrome and corneal tissue IL-6, TNF- α expression changes, compare the number of apoptotic ceils in the lacrimal gland. Methods： 180 white leghorn chicken, randomization, and start feeding. All animals were divided into normal control group, model group （ vitamin A deficient ） and .Schisandra group. The Schirmer I test （ SIT ） and tear film breakup time （ BUT ） were tested before treatment, after treatment two weeks and four weeks after treatment for each group Leghorn chickens. Fluorescein staining of the cornea and by immunohistochemistry IL-6 and TNF- α , lacrimal gland of each group number of apoptotic cells. Results ： Medication two weeks, four weeks later, compared with the model group, Schirmer test paper changed significantly longer wet, tear breakup time gradually increased, increased corneal surface moisture, a small amount of fluorescein dye coloring. Immunohistochemistry detected IL-6 and TNF-α were significantly lower in the group of Leghorn corneal epithelial cells, apoptosis were relieved lacrimal gland. Conclusion ： Schisandra can increase the secretion of tear, maintain the stability of tear film, its mechanism may he the inhibition of lacrimal gland cell apoptosis, inhibition of IL-6 and TNF- α expression, inflammatory reaction.