胃苓汤对肥胖小鼠嗜睡症状改善作用及机制探讨

Improving Effects and Mechanism Study of Weiling Decoction on Hypersomnia in Obese Mice

目的:观察胃苓汤对高脂饮食诱导的肥胖小鼠嗜睡现象的改善作用。方法:常规方法检测高脂饮食诱发肥胖小鼠的体重及睡眠时间,测定小鼠血清甘油三酯(TG)及游离脂肪酸(FFA)浓度,并监测胃苓汤灌胃处理后肥胖小鼠嗜睡情况以及血中TG和FFA的浓度变化。结果:高脂饮食组小鼠体重明显高于普通饮食组,并且活动期(夜晚)睡眠时间明显延长,血清TG与FFA浓度明显升高。高脂饮食动物改为普通饮食喂养20 d后,其睡眠时间及血清TG无显著变化,而血清FFA降低。灌胃胃苓汤可减少肥胖小鼠睡眠时间,同时降低血清中FFA及TG浓度。结论:高脂饮食喂养可致动物肥胖及嗜睡,并伴有血清TG和FFA浓度的升高。胃苓汤可以改善肥胖动物嗜睡症状并降低其血中TG和FFA浓度,推测其改善小鼠嗜睡症状的机制是通过降低血脂而完成的。

Objective ： To observe the improving effect of Weiling Decoction on the hypersomnia of obese mice induced by high-fat diet. Methods ： The weight, the total sleep time and the concentration of triglyceride （ TG ） and free fatty acids （ FFA ） in blood as well as the changes in these parameters by oral administration of Weiling Decoction were investigated through conventional methods in obese mice induced by high-fat diet. Results ： Comparing with normal diet group mice, the weight, the total time of sleep and the concentration of TG and FFA in high fat diet group mice significantly increased. 20 days after recovering back to the normal diet feeding, the sleep time and the blood TG concentration did not change obviously, only the FFA concentration of blood decreased in the obese mice. But the time of sleep during the activity period and the concentration of TG and FFA in blood were significantly decreased by oral administration of Weiling Decoction in obese mice. Conclusion： High fat diet lead animal to obesity and lethargy, and accompanying the high concentration of blood TG and FFA. Weiling Decoction can improve sleepiness symptoms and reduce the TG and FFA concentration levels of blood in obese animals. The mechanism of improving the sleepiness symptoms by Weiling Decoction in obese mice can he considered to relate to reducing the blood lipids.