3台不同型号的血细胞分析仪检测结果的比对分析与评价

Analysis and evaluation of test results acquired from three different types of hematology analyzers

目的对Sysmex公司生产的3台不同型号血细胞分析仪进行结果比对分析,探讨3台仪器检查结果的可比性及偏差是否在允许的范围内。方法取Sysmex XE2100、Sysmex XN3000、Sysmex XT4000i3台血细胞分析仪30d的室内质控结果进行分析。以多次参加全国室间质评成绩优秀的XE2100血细胞分析仪为参考仪器,以Sysmex XN3000血细胞分析仪和Sysmex XT4000i血细胞分析仪为比对仪器。每天随机选取低、中、高值患者标本4例共检测5d,对白细胞(WBC)、红细胞(RBC)、红细胞比积(HCT)、血红蛋白(HGB)、血小板(PLT)的结果进行比对分析,通过F检验、回归方程、相关系数来判断检测结果的准确性和可比性。结果 3台仪器的WBC、RBC、HCT、HGB、PLT检测结果差异无统计学意义(P>0.05),各项目测定结果间相关性密切(r≥0.975),偏差符合相关要求,具有可比性。结论在使用多台仪器检测时,要注重加强实验室仪器的质量控制,定期对不同的检测仪器进行校准和比对,以确保检测结果的准确性和一致性,从而更好地为临床服务。

Objective To perform the comparative analysis on the test results acquired from three different types of hematology analyzers,to explore their results comparability or whether their deviations being within the allowable range.Methods The 30 dinternal quality control results collected from the three types of analyzers,Sysmex XE2100,Sysmex XN3000 and Sysmex XT4000 i,were analyzed and analyze.The XE2100 hematology analyzer with excellent results in repeatedly participating in the national external quality control assessment was taken as the reference instrument,while the Sysmex XN3000 and Sysmex XT4000 ianalyzers served as the contrastive instruments.Four blood samples from different patients with high,medium and low values were randomly selected and detected for 5d.The results of white blood cell（WBC）,red blood cell（RBC）,hematocrit（HCT）,hemoglobin（HGB）and platelet（PLT）were performed the comparative analysis.Then the accuracy and comparability of the detection results were judged by Ftest,regression equation and correlation coefficient.Results The detection result of WBC,RBC,HCT,HGB and PLT had no statistical differences among three instruments（P〉0.05）.The correlation of various indexes had close correlation（r≥0.975）.The deviations conformed to the related requirements with comparability.Conclusion When conducting detection by multiple instruments,the quality control of experimental instrument should be paid attention to,the different detection instruments should be conducted the calibration and comparison at regular intervals for ensuring the accuracy and consistency of detection results,thus better serve clinic.