壮药龙利叶茎HPLC指纹图谱研究

HPLC Fingerprint of Stem of Sauropus rostratus Miq.

采用高效液相色谱法建立了壮药龙利叶茎的HPLC指纹图谱。色谱柱为Agilent Eclipse XDB-C18柱(250mm×4.6mm,5μm),以乙腈-0.08%醋酸溶液(体积比)为流动相,梯度洗脱,检测波长为280nm,柱温为25℃,流速为1mL/min,采用《中药色谱指纹图谱相似度评价系统》(2004A版)进行分析。结果表明10批龙利叶茎的HPLC指纹图谱共标定14个共有指纹峰,相似度均达到0.9以上,为建立龙利叶质量控制方法提供了科学依据。

High performance of liquid chromatography （HPLC） was adopted to study the HPLC fingerprint of stem of Sauropus rostratus Miq. The separation was performed on Agilent Eclipse XDB- C18 column with acetonitrile - 0. 08% acetic acid solution as mobile phase using a gradientelution, the detection wavelength was 280 nm, the column temperature was set at 25℃ and the flow rate was 1 mL·min^-1, the data calculation was performed with similarity evaluation system for chromatographic fingerprint of TCM （Version 2004A）; The results showed that in the fingerprint, 14 common peaks were confirmed and the similarity of 10 batches of Stem of Sauropus rostratus Miq.were above 0.9. The experiment result could provide scientific foundation for the quality control method of Sauropus rostratus Miq.