高效液相色谱法测定油茶籽壳水热碳化液中的3种糠醛类物质

HPLC Determination of 3 Furfurals in Hydrothermal Carbonization Solution of Camellia Oleifera Shells

采用高效液相色谱法同时测定油茶籽壳水热碳化液中的5-羟甲基糠醛、糠醛和5-甲基糠醛的含量。油茶籽壳水热碳化液经Thermo ODS色谱柱分离,以甲醇-水混合液作为流动相进行梯度洗脱,采用二极管阵列检测器,检测波长为285 nm。3种糠醛类物质的质量浓度在一定范围内与其峰面积呈线性关系,5-羟甲基糠醛、糠醛和5-甲基糠醛的检出限(3S/N)分别为0.02,0.005,0.02 mg·L^(-1),测定下限(10S/N)分别为0.04,0.02,0.05 mg·L^(-1)。加标回收率分别在74.9%~101%,94.5%~109%和98.6%~112%之间,测定值的相对标准偏差小于10%。

HPLC was applied for the determination of 5-hydroxymethyl-2-furfural, 2-furaldehyde and 5- methyl-2-furaldehyde in hydrothermal carbonization solution of camellia oleifera shells. The solution was separated on a Thermo ODS chromatographic column, with a mixture of methanol and water as mobile phase for gradient elution. DAD detector was used with the detection wavelength of 285 nm. Linear relationships were found between the peak areas and the mass concentrations of the 3 furfurals in definite ranges. The detection limits （3S/N） of 5- hydroxymethyl-2-furfural, 2-furaldehyde and 5-methyl-2-furaldehyde were 0. 02, 0. 005, 0. 02 mg ·L^-1, respectively, and the lower limits of determination （10S/N） were 0. 04, 0. 02, 0. 05 mg ·L^-1, respectively. Recovery rates obtained by standard addition method were in the ranges of 74. 9%- 101%, 94. 5%- 109% and 98. 6%-112%, respectively, and RSDs were less than 10%.