全血与血浆标本CRP检测的对比分析

Comparative Analysis of CRP Detection in Whole Blood and Plasma Samples

目的:探讨用静脉血在全血模式与血浆模式下检测C反应蛋白结果的可比性以及相关性。方法:随机选择2014年11月-2015年3月笔者所在医院门诊及住院患者141例,抽EDTA-K2抗凝血各2管,其中一管用于全血检测CRP的含量,另外一管用3500 r/min离心5 min后,取血浆用于检测血浆中CRP的含量,根据全血检测的CRP的浓度,将数据分为低值、中值、高值组。所得数据进行统计学分析。结果:将三组数据分别进行t检验比较,差异均无统计学意义(P>0.05)。在低、中、高值组中全血CRP与血浆CRP之间呈良好的相关性,r分别为0.98、0.89、0.81;且全血CRP的值与血浆CRP的值之间有良好的回归关系(P<0.01)。结论:用全血与血浆标本检测CRP结果对比无显著性差异,且全血CRP与血浆CRP有良好的相关性及线性回归关系。用全血标本检测CRP更适合于门诊与急诊,即用于初步的诊断。

Objective：To investigate the comparability and correlation between the results of C reactive protein detected by venous blood in whole blood and plasma.Method：From November 2014 to March 2015 in our hospital outpatient and inpatient 141 cases were randomly selected,2 EDTA-K2 anticoagulant tube were pumped,in which a tube for detection of the blood CRP content,another tube was centrifuged by 3500 r/min for 5 min,the plasma was used for the detection of plasma CRP levels,according to the detection of the blood CRP concentration,data for low value,median and high value group.The data were used for statistical analysis.Result：The data in the three groups were compared with t test,the differences were not statistically significant（P〉0.05）.In the low,middle and high value group,there was a good correlation between whole blood CRP and plasma CRP,the r were 0.98,0.89,0.81,and the value of whole blood CRP and plasma CRP value had a good regression relationship（P〈0.01）.Conclusion：There is no significant difference in the results of CRP with whole blood and plasma samples,and the whole blood CRP and plasma CRP had good correlation and linear regression.CRP is more suitable for outpatient and emergency department,that is for the initial diagnosis.