CTGF、TGF-β1和VEGF在不同分级肝硬化患者中的表达及临床意义

CTGF,TGF-β1 and VEGF Expression and Clinical Significance in Patients with Cirrhosis of Different Grade

目的:探讨肝硬化患者血清结缔组织生长因子(CTGF)、转化生长因子(TGF-β1)和血管内皮生长因子(VEG)三者与Child-Pugh分级的相关性及其变化规律。方法:随机选取笔者所在医院188例肝硬化患者为观察组,其中肝硬化Child-Pugh分级是A级的患者为A组(n=64例)、肝硬化Child-Pugh分级是B级的患者为B组(n=64例)和肝硬化Child-Pugh分级是C级的患者为C组(n=60例),同期选择100例健康体检者作为对照组,采用酶联免疫吸附实验(ELISA)检测各组血清CTGF、TGF-β1和VEGF及血清透明质酸(HA)、Ⅳ型胶原(Ⅳ-C)、层粘蛋白(LN)和Ⅲ型前胶原(PCⅢ),对其结果进行比较分析。结果:观察组患者血清CTGF、TGF-β1和VEGF水平均明显高于对照组(P<0.05),随着Child-Pugh分级的逐渐递增,血清CTGF、TGF-β1和VEGF水平呈进行性升高(P<0.05),血清CTGF、TGF-β1和VEGF水平与血清透明质酸(HA)、Ⅳ型胶原(Ⅳ-C)、层粘蛋白(LN)和Ⅲ型前胶原(PCⅢ)呈正相关(P<0.01)。结论:肝硬化患者血清CTGF、TGF-β1和VEGF水平高于健康照组,而且随着肝硬化病程的发展,血清CTGF、TGF-β1和VEGF逐渐升高,与肝纤维化标志物呈正相关,CTGF、TGF-β1和VEGF对评估肝硬化严重程度和判断预后具有较高的临床价值。

Objective：To explore the correlation of between connective tissue growth factor（CTGF）,transforming growth factor（TGF-β1） and vascular endothelial growth factor（VEG） with the three Child-Pugh classification and its variation in patients with cirrhosis.Method：In our hospital,188 patients with cirrhosis were randomly selected as the observation group,which cirrhosis Child-Pugh classification was A-class as group A（n=64 patients）,liver cirrhosis patients with Child-Pugh Class B classification was as group B（n=64 patients） and cirrhosis Child-Pugh class C classification was for group C（n=60 patients）,100 healthy control people in the same period were selected as the control group,serum CTGF,TGF-β1 and VEGF in each group checked by enzyme-linked immunosorbent assay（ELISA）,and hyaluronic acid（HA）,Ⅳ collagen（Ⅳ-C）,laminin（LN）,Ⅲ procollagen（PCⅢ）,the results were compared.Result：Serum CTGF,TGF-β1 and VEGF levels in the observation group were significantly higher than those in the control group（P〈0.05）,with the Child-Pugh classification incrementally,serum CTGF,TGF-β1 and VEGF levels showed a progressive increased（P〈0.05）,serum CTGF,TGF-β1,VEGF serum levels and hyaluronic acid（HA）,Ⅳ collagen（Ⅳ-C）,laminin（LN）,Ⅲ procollagen（PCⅢ） were positively correlation（P〈0.01）.Conclusion：Serum CTGF,TGF-β1 and VEGF levels in patients with cirrhosis are higher than the healthy control group,and with the course of development of cirrhosis,serum CTGF, TGF-β1 and VEGF increased gradually,and they are positively correlated with liver fibrosis markers,CTGF,TGF-β1 and VEGF have high clinical value to evaluate the severity of liver cirrhosis and prognosis.