鸡爪槭花粉活力与贮藏特性的研究

Research on Pollen Germination and Pollen Preservation Characteristics of Acer palmatum

以鸡爪槭花粉为试材,采用液体培养基法进行离体培养,研究鸡爪槭花粉活力及贮藏特性。结果表明,鸡爪槭花粉萌发观察的最佳培养时间为24 h;不同浓度蔗糖、硼酸对花粉萌发具有显著影响,确定了最适宜鸡爪槭花粉萌发的液体培养基为15%蔗糖+0.01%硼酸。不同的贮藏条件对花粉萌发有显著的影响,且花粉活力随着贮藏时间的延长而下降;室温条件下贮藏鸡爪槭花粉15 d之后完全丧失萌发能力,冷藏处理的花粉在20 d后花粉活力降至28.1%,而冷冻贮藏的花粉贮藏20 d以后花粉活力仍为46.6%。冷藏(4℃)和冷冻(-20℃)有利于保存鸡爪槭花粉,如花粉保存15 d之内,冷藏和冷冻条件均可以;如保存15 d以上,则冷冻保存更合适。

The pollen vitality and storage capacity of Acer palmatum were characterized by using in vitro culture. The result showed that the optimal pollen culture time was 24 hours. Sucrose and boric acid affected pollen viability remarkably,and the optimum culture solution was15 % sucrose ＋ 0. 01 % H_3BO_3. Different storage conditions had great effects on the germination of pollen,and pollen viability decreased with the extension of storage time. The pollen viabilities under natural conditions decreased very fast,and reached 0 after 15 days,while those of under the temperature of 4 ℃ decreased slower,and still maintained 28. 1 % and the pollen viabilities under the temperature of-20 ℃ maintained 46. 6 % after 20 days. Therefore,the temperature of 4 ℃ and- 20 ℃ were suitable for storage capacity of A. palmatum such as for pollen stored in the 15 d,the refrigeration and freezing conditions were both possible; If kept for more than 15 days,then frozen condition was more appropriate.