不同地区灰霉病菌株对腐霉利的抗性测定及生理生化研究

Study on Resistance,Physiological and Biochemical Effects of Procymidone against Different Districts Strains of Botrytis cinerea

采用菌丝生长速率抑制法测定了北京、山东、江西等7个地区31株灰霉菌株和1株实验室标准菌株（BO5.10）对腐霉利的毒力。结果表明,32个供试灰霉菌株中,8个菌株对腐霉利属中等抗性,20个菌株属于低等抗性,4个菌株为敏感菌株。进一步测定了个别抗性菌株与敏感菌株的高盐渗透压、甘油含量以及电导率之间的差异。供试抗性及敏感菌株在1.25~10 g/L Na Cl浓度范围内可刺激菌落生长,超过此浓度界线抑制菌落生长,且对抗性菌株抑制率大于敏感菌株。抗腐霉利菌株甘油含量和电导率均高于敏感菌株。以上结果为灰霉病的抗性治理提供了理论依据。

The toxicities of 31 Botrytis cinerea strains were isolated from seven districts,including Beijing,Shandong,Jiangxi,and so on,and a standard strain from lab（ BO5. 10） against procymidone were assayed with mycelial growth-inhibition capacity. The results indicated that there were 8 middle-resistance,20 low-resistance and 4 sensitive strains. The differences of high salt permeability,content of glycerol and conductivity between resistance and sensitive strains of B. cinerea were further studied. The results suggested that the concentrations with1. 25 to 10 g / LNa Cl could promote the hypha growth. Nevertheless,the inhibitions were found when the concentrations surpassed 10 g /LNa Cl,of which resistance strains were stronger than those in the sensitive strains. Meanwhile,the content of glycerol and conductivity of resistant was higher than those of sensitive-strain. This results provide the theoretical basis for the resistance management of B. cinerea.