温胃调肠颗粒对腹泻型肠易激综合征大鼠的作用机制研究

Mechanism of Wenwei Tiaochang Granule on Rats with Diarrhea-Predominant Irritable Bowel Syndrome

目的探讨温胃调肠颗粒对腹泻型肠易激综合征大鼠的作用机制。方法 60只SD大鼠,随机分为对照组、模型组、阳性药组以及温胃调肠颗粒低、中、高剂量组。采用番泻叶灌胃,束缚和夹尾刺激14d,造成腹泻型肠易激综合征模型。给药14d后,取血、空肠和结肠,MTL测试盒检测血清、空肠匀浆中胃动素含量;RT-PCR法检测结肠组织中降钙素基因相关肽（CGRP）及P物质（SP）的mRNA水平,Western blot检测CGRP和SP的蛋白表达水平;取胸腺、脾脏,称质量,计算脏器指数。结果模型组大鼠体质量增长缓慢,摄食量下降,胸腺、脾指数,血浆和空肠匀浆组织中MTL含量显著降低,结肠的CGRP、SP mRNA和蛋白表达水平显著升高,与对照组比较有显著性差异（P〈0.05~0.01）。温胃调肠颗粒高、中剂量可以显著升高胸腺、脾指数,血浆和空肠匀浆组织中MTL含量,降低结肠的CGRP、SP mRNA和蛋白表达水平,与模型组比较有显著性差异（P〈0.05~0.01）。结论温胃调肠颗粒可以通过调节机体MTL含量,CGRP、SP mRNA和蛋白表达水平来治疗腹泻型肠易激综合征。

OBJECTIVE To explore the effects and mechanisms of Wenwei Tiaochang Granule on diarrhea-predominant irritable bowel syndrome rats.METHODS 60 SD rats,were randomly divided into control group,model group,positive drug group,and low,middle and high dose groups of Wenwei Tiaochang Granule.In order to make the rats get diarrhea-predominant irritable bowel syndrome,the rats were administrated with senna,bound limbs and clipped tails for 14 d.After 14 dof administration with positive drug and Wenwei Tiaochang Granule,the blood,jejunum,colon,thymus and spleen were collected.Motilin assay kit was used to detect motilin（MTL）content in plasma and jejunum.Use the weight of thymus and spleen to calculate their organ index.Calcitonin gene-related peptide（CGRP）and substance P（SP）mRNA level and protein expression level was detected by RT-PCR and Western-blot in colonic tissues.RESULTS Compared with the control group,the growth rate and food intake increased slower in model group,at the same time,the thymus index,spleen index and MTL content in plasma and jejunum decreased significantly,while the CGRP and SP mRNA and protein expression level of colon increased significantly（P〈0.05~0.01）.Compared with the model group,the middle and high dose groups of Wenwei Tiaochang Granule could significantly increase the thymus index,spleen index and MTL content in plasma and jejunum,and decrease CGRP and SP mRNA and protein expression level of colon（P〈0.05~0.01）.CONCLUSION By regulating the content of MTL and the expression level of CGRP and SP mRNA and protein,Wenwei Tiaochang Granule can be used to treat diarrhea-predominant irritable bowel syndrome.