天麻素对高糖条件下人晶状体上皮细胞氧化应激的影响

Effect of gastrodin against oxidative stress induced by high glucose in human lens epithelium cells

目的探讨天麻素对高糖诱导的人晶状体上皮(human lens epithelial,HLE)细胞氧化应激的影响。方法建立高糖诱导的HLE细胞氧化损伤模型,用不同浓度的天麻素干预,MTT检测细胞活力,流式细胞仪检测细胞凋亡率,透射电镜观察超微结构变化,比色法检测凋亡因子表达变化。结果 MTT结果显示用1.0×10-6mol·L^(-1)和1.0×10-5mol·L^(-1)天麻素处理后,HLE细胞活性分别提高到(62.00±3.74)%和(79.57±5.26)%,与氧化损伤组(45.42±4.14)%比较差异具有统计学意义(P<0.05);流式细胞计数结果显示预先给予1.0×10-6mol·L^(-1)和1.0×10-5mol·L^(-1)的天麻素作用后,HLE细胞凋亡率分别下降到(30.33±3.92)%和(13.45%±2.53)%,与氧化损伤组(43.83±4.25)%比较差异具有显著性(P<0.05);电镜观察结果显示伴随天麻素作用浓度的增加,细胞形态亦逐渐得到改善;此外,天麻素可以抑制高糖诱导的HLE内凋亡因子的表达,抑制效应与剂量正相关。电镜观察结果显示伴随天麻素作用浓度的增加,细胞形态亦逐渐得到改善。此外,天麻素可以抑制高糖诱导的HLE内凋亡因子的表达,抑制效应与剂量正相关。结论天麻素可以抑制高糖诱导的人HLE细胞的凋亡,对糖尿病性白内障具有预防作用。

Objective To investigate the effect of gastrodin on high glucose-induced human lens epithelial（ HLE） cells from oxidative stress. Methods High glucose induced HLE cells injury model was constructed and intervented with different concentrations of gastrodin,cells viability was observed by transmission electron microscopy,apoptosis factor expression were tested by colorimetric detection. Results MTT results showed that HLE cells activity increased to（ 62. 00 ± 3. 74） % and（ 79. 57 ± 5. 26） % after treated with gastrodine,the difference was statistically significant compared with oxidative injury group（ 45. 42 ± 4. 14） %（ P〈0. 05）; flow cytometry results showed that after pretreatment with 1. 0 × 10^-6mol·L^（-1）and 1. 0 × 10^-5mol·L^（-1）gastrodin,HLE apoptosis rate decreased to（ 30. 33 ± 3. 92） % and（ 13. 45 ± 2. 53） %,the difference was statistically significant compared with oxidative injury group（ 43. 83 ±4. 25） %（ P〈0. 05）; electron microscopy results showed that cell morphology gradually improved accompanied with gastrodine concentration increased; besides,gastrodin could inhibit the expression of high glucose-induced apoptotic factors in HLE cells and the inhibitory effect positively correlated with the dose. Conclusion Gastrodin can inhibit high glucose-induced apoptosis in human HLE cells,which has preventive effect on diabetic cataract.