1株耐镉木霉菌株的筛选鉴定

Screening and Identification of a Trichoderma Strain with Tolerance to Cadmium

为丰富微生物降解菌资源应用于重金属污染土壤的生物修复,采用土壤平板稀释法结合28SrDNA序列测定与系统发育树构建等方法,研究重金属镉污染土壤中强耐镉性菌株种类及其对Cd^(2+)的降解作用。结果表明:筛选到1株具有强耐镉性的菌株T61,该菌株为哈茨木霉菌(Trichoderma harzianum);在pH 4、温度28℃时,其对Cd^(2+)的降解率最大,为58%。

The species of strong Cd-tolerance strains and the Cd2＋degradation effect in heavy metal pollution soils were studied by soil plate dilution,28 S rDNA sequence determination and phylogenetic tree structure methods to enrich microbial strains resources for application of microbial degrading bacteria in remediation of soil contaminated with heavy metals.Results：A T61 strain with strong Cd-tolerance is screened and identified as Trichoderma harzianum.The Cd2＋degradation rate of T61 strain is up to 58%under the conditions of pH 4 and 28℃.