摘要
为丰富微生物降解菌资源应用于重金属污染土壤的生物修复,采用土壤平板稀释法结合28SrDNA序列测定与系统发育树构建等方法,研究重金属镉污染土壤中强耐镉性菌株种类及其对Cd^(2+)的降解作用。结果表明:筛选到1株具有强耐镉性的菌株T61,该菌株为哈茨木霉菌(Trichoderma harzianum);在pH 4、温度28℃时,其对Cd^(2+)的降解率最大,为58%。
The species of strong Cd-tolerance strains and the Cd2+degradation effect in heavy metal pollution soils were studied by soil plate dilution,28 S rDNA sequence determination and phylogenetic tree structure methods to enrich microbial strains resources for application of microbial degrading bacteria in remediation of soil contaminated with heavy metals.Results:A T61 strain with strong Cd-tolerance is screened and identified as Trichoderma harzianum.The Cd2+degradation rate of T61 strain is up to 58%under the conditions of pH 4 and 28℃.
出处
《贵州农业科学》
CAS
2016年第6期94-98,共5页
Guizhou Agricultural Sciences
基金
国家自然科学基金项目"辣椒抗根结线虫Me3候选基因功能验证及遗传解析"(31470105)
关键词
哈茨木霉
镉
重金属污染
微生物降解菌
Trichoderma harzianum
Cd
heavy metal pollution
microbial degrading bacteria