活菌制剂对2型糖尿病胃肠神经病变患者肠道菌群的影响

Effect of Living Bacteria Agent on Intestinal Flora in Type 2 Diabetes Mellitus Patients With Gastrointestinal Dysfunction

目的研究2型糖尿病并胃肠功能紊乱患者服用双歧杆菌三联活菌胶囊治疗前后肠道双歧杆菌的变化。方法选取30例2型糖尿病并胃肠神经病变患者,给予双歧杆菌三联活菌胶囊口服,每次2粒,3次/d,治疗1个月,治疗前后收集粪便样品,提取样品中细菌DNA,根据双歧杆菌总菌的16S r RNA序列设计特异性引物,并应用实时荧光定量PCR技术对60份粪便样品中的双歧杆菌总菌进行定量检测。结果2型糖尿病并胃肠功能紊乱患者服用双歧杆菌三联活菌胶囊治疗后肠道双歧杆菌总菌数量较治疗前增加(P<0.05)。结论 2型糖尿病伴胃肠功能紊乱患者口服双歧杆菌活菌制剂能有效增加肠道双歧杆菌数量,改善胃肠道症状,双歧杆菌活菌制剂在2型糖尿病伴胃肠功能紊乱患者的治疗上有较好的运用前景。

Objective To study the influence of intestinal bifidobacteria on the type 2 diabetes patients with gastrointestinal dysfunction after taking bifid triple viable capsule.Methods Selecting 30 cases of type 2 diabetes patients with gastrointestinal dysfunction. Taking the bifid triple viable capsule（2 pieces each time,three times a day）for a month. Extracting the bacterial DNA of the fecal samples before and after the treatment. Designing the specific primer of bifidobacteria according to the bacterial 16SrRNA sequence. Quantitatively testing the total volume of bifidobacteria on the two different groups by applying the real-time PCR technology.Results After taking the bifid triple viable capsules,the total amount of bifidobacteria increased compared to the previous case（P〈0.05）for the type 2 diabetes patients with gastrointestinal dysfunction.Conclusion Bifid triple viable capsules help increase the amount of intestinal bifidobacterium effectively and improve the gastrointestinal symptoms. It is promising for type 2 diabetes patients with gastrointestinal dysfunction by using bifid triple viable capsules as the medical treatment.