献血者核酸筛查情况分析

目的分析枣庄市献血者的核酸检测情况,为以后更好的开展核酸检测工作提供依据。方法 ALT检测合格及酶联免疫法检测乙肝、丙肝、艾滋病、梅毒阴性的献血者标本9901例,进行HBV-DNA、HCV-RNA和HIV-RNA的联合混样检测,对混样阳性的标本再进行拆分检测。并对拆分乙肝HBV-DNA阳性的样本进行乙肝两对半血清学的酶联免疫检测。结果共完成9901例标本的核酸混样检测,拆分阳性的标本7例,阳性率为0.071%(7/9901)。HBc Ab阳性或HBc Ab阳性伴HBs Ab强弱阳性占71.40%(5/7),而HCV-RNA、HIV-RNA均阴性。对7例HBV-DNA阳性的标本进行乙肝两对半检测,结果显示:HBc Ab阳性2例(28.5%),HBc Ab阳性伴HBs Ab强弱阳性3例(42.9%),HBs Ag弱阳性伴HBs Ab阳性1例(14.3%),两对半结果全部阴性1例(14.3%)。结论常规检测后的献血者依然有传染性的可能,核酸检测可有效降低传染性的风险。

Objective Analysis of NAT in Zaozhuang blood donors, provide the basis for better development of NAT work. Methods Qualified ALT and ELISA in detection of hepatitis B, hepatitis C, syphilis and HIV negative blood specimens, HBV-DNA and HCV-RNA and HIV-RNA combined with separation detection of mixed samples and the positive samples. And ELISA of two pairs of semi-hepatitis, the sample is HBV-DNA positive. Results This study conduct 9901 mix sample test in total, 7 samples are split positive, and the ratio is 0.071% （7/9901）, while HCV-RNA and HIV-RNA are negative. Two pairs of semi-hepatitis test of 7 HBV-RNA samples showed that, 2 of them are HBcAb positive （28.5%）, 3 of them are HBcAb positive with HBsAb weak positive （42.9%）, 1 of them is HbsAg weak positive with HBsAb positive（14.3%）, and 1 is negative （14.3%）. Conclusion After the routine detection of blood donors is still contagious, NAT can effectively reduce the risk.