γ射线照射人肝细胞对BMP信号通路相关基因表达的影响

Effects of gamma ray on the expression of BMP pathway related genes in human hepatocytes

利用实时荧光定量PCR技术对人肝细胞株受不同剂量(0.1、0.2、0.5、1.0、2.0、4.0 Gy)^(60)Coγ射线照后不同时间点(6、12、24 h)的骨形成蛋白(BMP)信号通路相关基因表达的剂量和时间效应关系进行研究。结果显示,不同剂量照后不同时间点,BMP信号通路相关基因骨形成蛋白2(BMP2)、骨形成蛋白7(BMP7)、骨形成蛋白受体1A(BMPR1A)、骨形成蛋白受体1B(BMPR1B)、骨形成蛋白受体2(BMPR2)的溶解曲线均为单峰,均为特异性扩增产物。在不同照射剂量点BMP2的表达水平与照后时间呈正相关;BMP7在0.1、0.2、0.5 Gy的表达水平与照后时间呈负相关,在照后12和24 h的表达水平与照射剂量呈正相关;BMPR1A在0.1、0.2、1.0 Gy的表达水平与照后时间呈正相关;BMPR1B在0.2、0.5、1.0 Gy的表达水平与照后时间呈正相关,在4.0 Gy的表达水平与照后时间呈负相关;BMPR2在照后12 h的表达水平与照射剂量呈负相关。

To study the irradiation effects at different doses （0.1, 0.2, 0. 5,1. 0,2. 0,4. 0 Gy） and different time （6,12,24 h） on the expression of BMP pathway related genes in human hepatocytes by real -time quantitative PCR. Results Solution curves of B -actin, BMP2, BMP7, BMPR1A, BMPR1B, and BMPR2 with different doses and time were single peak, showed the specific amplification. The expression levels of BMP2 were positively correlated with time; the expression levels of BMP7 werenegatively correlated with time at doses of 0.1, 0.2, and 0.5 Gy, and positively correlated with dose at 12 and 24 h after irradiation; the expression levels of BMPR1A were positively correlated with time at doses of 0.1, 0.2, and 1.0 Gy; the expression levels of BMPR1B were positively correlated with time at doses of 0.2, 0.5, and 1.0 Gy, and negatively correlated with time at dose of 4.0 Gy; the expression levels of BMPR2 were negatively correlated with dose at 12 h after irradiation.