补肾活血方对多囊卵巢综合征伴不孕症患者卵巢颗粒细胞IGF-1R m RNA的影响

Effect of Ovarian Granulosa Cell IGF- 1R m RNA of Application of Bushenhuoxue Prescription Treatment for Infertile Women with Polycystic Ovary Syndrome

目的探讨补肾活血方对多囊卵巢综合征伴不孕症患者卵巢颗粒细胞IGF-1R m RNA的影响。方法选取2010年3月—2014年3月静宁县人民医院诊治多囊卵巢综合征伴不孕症患者70例,随机分为观察组和对照组,每组各35例。对照组患者给予常规促排卵治疗,观察组患者在对照组治疗的基础上给予补肾活血方治疗,疗程为6个月经周期。观察并记录两组患者在相应方法干预后血清雌二醇(E2)、睾酮(T)、黄体生成素/卵泡雌激素(LH/FSH)和中医症候积分的变化情况;测定取卵日卵巢颗粒细胞IGF-1R m RNA的表达情况。结果治疗前,两组患者的上述观察指标相比,差异无统计学意义(P>0.05);治疗后,两组患者的E2升高,T、LH/FSH和中医症候积分下降,其中观察组患者的改善程度优于对照组,差异有统计学意义(P<0.05);观察组患者卵巢颗粒细胞IGF-1R m RNA的表达显著高于对照组,差异有统计学意义(t=3.938,P=0.000)。结论对多囊卵巢综合征伴不孕症患者给予补肾活血方治疗,可以提高IGF-1R m RNA的表达水平,促进卵泡发育,调节机体的黄体功能,提高治疗效果,值得临床推广。

OBJECTIVE To discuss the effect of ovarian granulosa cell IGF-1R m RNA of application of bushenhuoxue prescription treatment of infertile women with polycystic ovary syndrome. METHOD 70 cases of infertile women with polycystic ovary syndrome treated in our hospital from March 2010 to March 2014 were randomly divided into observation group and control group （35 cases in each group）. The control group was given conventional ovulation induction treatment, and the observation group was given bushenhuoxue prescription treatment on the basis of the control group, the period of treatment was 6 menstrual cycle. Serum estradiol（E2）, testosterone（T）, luteinizing hormone/follicle estrogen（LH/FSH）, TCM symptom scores, the expression of ovarian granulosa cell IGF-1R m RNA of the two groups after treatment were observed. RESULTS The differences of the observation index before treatment were not statistically significant（P〉0.05）. After treatment, E2 increased, T, LH/FSH, TCM symptom scores decreased in the two groups, and the improvement of the observation group was better than that of the control group, the differences were statistically significant （P〈0.05）. The expression of ovarian granulosa cell IGF-1R m RNA of the observation group was significantly higher than the control group, the differences were statistically significant（t=3.938, P=0.000）. CONCLUSION Application of bushenhuoxue prescription treatment of infertile women with polycystic ovary syndrome cluld increase the expression of IGF-1R m RNA, promote follicular development, regulate the luteal function, improve the therapeutic effect, which was worthy of popularization.