氨基化二氧化硅荧光微球的制备及其表征

Preparation and characterization of silica fluoresent microspheres with amino groups

首先合成了荧光量子产率高的不对称罗丹明衍生物,以其为荧光染料,正硅酸乙酯为硅源,在酸性条件下,运用包埋法制备了二氧化硅荧光微球。然后采用硅烷偶联剂氨丙基三乙氧基硅烷,在二氧化硅荧光微球表面引入氨基功能基团。通过扫描电镜、荧光显微镜、红外光谱仪、荧光分光光度计对氨基化二氧化硅荧光微球的形貌、结构和荧光性能进行了表征。结果表明,氨基化二氧化硅荧光微球具有良好的单分散性,粒径分布窄,荧光性能好且稳定,并成功的引入氨基基团。

Asymmetrical rhodamine derivative was firstly synthesized with a yield of high fluorescence quantum. Using asymmetrical rhodamine derivative as fluoresent dye,tetraethyl orthosilicate as the raw material,we prepared silica fluoresent microspheres by embedding method under acidic solutions. Then surface modification on the silica fluoresent microspheres by aminopropyl triethoxysilane was carried out. Finally,silica fluorescent microspheres with amino groups were characterized by scanning electron microscopy, fluorescence microscopy, fourier transform-infrared spectroscopy and fluorescence spectrophotometry. The results indicated that the obtained silica fluorescent microspheres were of good dispersion,narrow particle size,stable and high fluorescence intensity. And amino groups were successfully introduced on the surface.