不同产地冬虫夏草对小鼠免疫功能的影响

Effect of Cordyceps Sinensis from Different Origins on Immune Response in Mice

目的考察不同产地冬虫夏草对小鼠免疫功能的影响。方法将青海产和西藏产冬虫夏草制备成粉末,分别设置大、中、小(0.4,0.2,0.1 g·kg^(-1))剂量组,另设正常对照组(纯化水)。观察两个产地冬虫夏草粉末对刀豆蛋白A(Con A)诱导的小鼠脾淋巴细胞增殖功能,二硝基氟苯诱导的小鼠迟发型变态反应(DTH)、抗体生成细胞数、血清溶血素水平,以及小鼠碳廓清能力、小鼠腹腔巨噬细胞吞噬荧光微球和自然杀伤(NK)细胞活性的影响。结果两个产地冬虫夏草粉末大、中剂量组小鼠脾淋巴细胞增殖功能、碳廓清能力、腹腔巨噬细胞吞噬荧光微球及NK细胞活性均显著提高,与正常对照组比较,差异有统计学意义(P<0.05);与正常对照组比较,两个产地冬虫夏草粉末中剂量组小鼠抗体生成细胞数均明显增加,大剂量组出现抑制(P<0.05);青海产冬虫夏草粉末中、大剂量组小鼠DTH显著提高,青海产冬虫夏草粉末大剂量组与西藏产冬虫夏草各剂量组比较,差异有统计学意义(P<0.05);与正常对照组比较,青海产冬虫夏草粉末中、大剂量组及西藏产冬虫夏草中剂量组小鼠血清溶血素水平明显提高(P<0.05),两种冬虫夏草粉末中、大剂量组间差异有统计学意义(P<0.05)。结论青海和西藏产冬虫夏草粉末均能明显提高小鼠免疫功能,两个产地冬虫夏草粉末增强免疫功能作用相当。

Objective To investigate the effect of cordyceps sinensis from different origins on immune response in mice. Methods Cordyceps sinensis from two origins were prepared into powder, and then the mice were divided into high, middle and low dose（0.4,0.2,0.1 g.kg-1）groups, respectively.In addition, purified water was given as the normal control group.Effects of cordyceps from two different origins were observed by detecting spleen lymphocyte proliferation induced by ConA, delayed type hypersensitivity （ DTH） in mice induced by sheep red blood cells （ SRBC ） , the number of antibody-producing cells, carbon clearance and peritoneal macrophages Swallow fluorescent microspheres, as well as the activity of NK cells. Results The ability of spleen lymphocyte proliferation induced by ConA, carbon clearance and peritoneal macrophages Swallow fluorescent microspheres, and the activity of NK cells were significantly enhanced in the middle and high dose group of two different origins cordyceps, compared with normal control group （P〈0.05）.Additionally, the number of antibody-producing cells was obviously increased in medium dose group of both origins cordyceps and decreased in the high dose group （P〈0.05）.The middle and high dose Qinghai cordyceps significantly improved DTH in mice, while Tibet cordyceps sinensis had no obvious effect, and there was significant difference （P〈0.05） between the high dose group of Qinghai and three dose groups of Tibet Cordyceps sinensis.In addition, levels of serum hemolysin in mice were significantly increased in the middle and high dose group of Qinghai and high dose group of Tibet Cordyceps sinensis （P〈0.05）, and the differences of corresponding medium and high doses of two origins were significant （ P〈0.05） . Conclusion Cordyceps sinensis of both different regions significantly improved the immune response of mice.However, the efficacy between the two origins was roughly equivalent and had no significant difference.