川芎与细辛挥发油β-环糊精包合物成分分析及经皮渗透实验

Transdermal Penetration Test of Four Preparations Containing Volatile oil Extracted From Chuanxiong Rhizoma and Asari Radix et Rhizoma in Vitro and Its β-cyclodextrin Inclution Complex

目的考察川芎和细辛挥发油β-环糊精(β-CD)包合物及其凝胶膏剂经皮渗透性能。方法将川芎和细辛挥发油、川芎和细辛挥发油β-CD包合物、川芎和细辛挥发油β-CD包合物凝胶膏剂以及川芎和细辛挥发油与β-CD混合物的凝胶膏剂等4种制剂涂敷于SD大鼠皮肤,以40%乙醇为吸收液,采用Franz扩散装置进行体外透皮实验。气相色谱-质谱(GC-MS)法测定透皮吸收液中挥发油成分,考察其经皮渗透的差异。结果 4种含川芎和细辛挥发油制剂的经皮渗透速率常数依次为:挥发油直接涂皮肤上>挥发油β-CD包合物凝胶膏剂>挥发油β-CD包合物直接涂皮肤上>β-CD和挥发油混合物凝胶膏剂。结论川芎和细辛挥发油用β-CD包合后,成分无明显改变,包合物凝胶膏剂的经皮渗透性能优于未包合挥发油的凝胶膏剂。

Objective To investigate the influence of β-cyclodextrin （β-CD ） inclusion and its cataplasma on the transdermal permeability of volatile oil extracted from Chuanxiong Rhizoma and Asari Radix et Rhizoma by means of percutaneous penetration test of four preparations containing of the volatile oil. Methods Using the modified Franz diffusion pool as equipment,with 40% ethanol solution as absorption solution and SD rat skin coated with the volatile oil,aβ-CD inclusion complex of the volatile oil,a cataplasma of theβ-CD inclusion complex of the volatile oil,and a cataplasma of the mixture of the volatile oil andβ-CD,respectively,as experimental skin,the transdermal penetration test was carried out.The cumulative penetrative amounts of volatile oil in the absorption liquid were measured by GC-MS in order to evaluate the differences in the percutaneous permeability. Results The percutaneous penetration rate constant size order of four preparations containing the volatile oil is as follows：the volatile oil directly coated on the skin 〉 the cataplasma of β-CD inclusion complex of the volatile oil 〉 the β-CD inclusion complex of the volatile oil directly coated on the skin 〉 the cataplasma of the mixture of the volatile oil and β-CD. Conclusion The compositions of the volatile oil retrieved from the β-CD inclusion complex are no significant changes. The percutaneous permeability of the cataplasma of β-CD inclusion complex of the volatile oil is better than that of the cataplasma of the mixture of volatile oil and β-CD.