摘要
目的:完善扶正化瘀胶囊的质量标准。方法:建立了其主要组分丹参、桃仁和五味子的薄层层析鉴别方法;采用反相液相色谱法(RP-HPLC),在C_(18)色谱柱上,以甲醇-乙腈-水-甲酸(30:10:59:1)为流动相,检测波长286 nm,测定了丹酚酸B的含量。结果 :薄层图谱斑点清晰,空白对照无干扰。丹酚酸B在4.83-145.05μg/ml范围内有较好的线性关系。平均回收率(n=9)为99.33%,RSD为1.76%。结论 :本方法操作简便、快速,结果可靠,重复性好,可作为该产品的质量控制方法。
Objective: To improve the quality standard for Fuzheng Huayu capsules. Methods: Salviae miltiorrhizaea, Persicae semen, Schisandrae chinensis fructus in these capsules were identified by TLC. The contents of salvianolic acid B were determined by RP-HPLC on a C_(18) column with a mobile phase of methanol-acetonitrile-water-formic acid(30:10:59:1) at wavelength of 286 nm. Results: The spots on TLC were clear and accurate. The standard curve of salvianolic acid B was linear over the range of 4.83~145.05 μg/ml with an average recovery of 99.33%(RSD 1.76%). Conclusion: The methods are convenient, reliable and accurate for the quality control of Fuzheng Huayu capsules.
出处
《上海医药》
CAS
2016年第13期29-32,47,共5页
Shanghai Medical & Pharmaceutical Journal