肉牛宰前运输应激对其血液理化指标及免疫机能的影响

Effects of preslaughter transportation stress on blood physiological and biochemical indexes and immunity function of beef cattle

选择20头体质量500kg左右的西门塔尔杂交牛进行试验,研究肉牛宰前运输应激对其血液理化指标及免疫机能的影响。结果表明:与运输前相比,运输后体质量极显著降低(P<0.01),体温极显著升高(P<0.01);血清谷草转氨酶(P<0.01)、肌酸激酶(P<0.05)、乳酸脱氢酶(P<0.05)和碱性磷酸酶(P<0.05)水平均显著升高;血清葡萄糖(P<0.01)和乳酸(P<0.05)含量显著升高;尿素氮、白蛋白、总胆固醇、甘油三酯、牛羟丁酸、非酯化脂肪酸和总蛋白运输前后变化不大(P>0.05),血清pH值、钾、钠、钙及氯离子浓度无显著变化(P>0.05);血清皮质醇水平显著上升(P<0.05),促肾上腺皮质激素及肾上腺素水平运输前后均差异不显著(P>0.05);血清IgM、IgG水平均显著升高(P<0.05),C-反应蛋白(P<0.01)IL-6(P<0.05)和干扰素(IFN-γ)(P<0.05)显著降低,血清IgA、IL-1β和肿瘤坏死因子运输前后均变化不大(P>0.05)。

The experiment was condarcted to study the effects of preslaughter transportation stress on blood physiological and biochemical indexes and immunity function of beef cattle.Twenty 500kg-BW Simmental crossbred cattles were randomly selected.The results showed that compared with the values before transportation,BW was decrereased（P〈0.01）,the body temperature was significantly raised（P〈0.01）;AST（P〈0.01）,CK（P〈0.05）,LDH（P〈0.05）and ALP were significantly raised.Glu（P〈0.01）and Lac（P〈0.05）were significantly raised.BUN,ALB,Tc,TG,OHB,NEFA and TP were not changed obviously（P〈0.05）.pH,K,Na,Ca and CI were not changed obviously.COR was significantly raised（P〈0.05）,ACTH and EPI were not changed obviously（P〈0.05）.IgM and IgG were significantly raised（P〈0.05）,CRP（P〈0.01）,IL-6（P〈0.05）and IFN-γ（P〈0.05）were significantly decrereased,IgA,IL-1βand TNF-βwere not changed obviously（P〈0.05）.