摘要
摘要：研究高速自动化精准拾取及放置单个细胞。在本系统中，首先控制系统将微量玻璃管放置在内有细胞的微孔上面．并提前利用原子力显微镜测量出玻璃管上端和微孔底部间的距离；然后用计算机控制微量玻璃管移动到期望位置，根据微量玻璃管与目标微孔间的距离位置，把细胞转到目标微孔中。在实验中，建立了一个基于电压反馈的操控系统，利用此系统可以判断出细胞是否被放置到目标微孔中。本实验方法能实现自动化操控微孔中细胞高效率的操控、转移和拾取。
An automation operation system for the single cell aspiration and placement with high throughput were pre- sented in this article. In the system, the control system was firstly utilized to move the mieropipette to the place above the microwell which contained the cell, and measure the current distance between the top of the micropipette and the bottom of the microwell device in advance by atomic force microscopy. Then we can move the micropipette to the cell of interest- ed region by the computer to control the motor and aspire and transfer it to the target microwell for placement according to the record in the computer about the position relation between the micropipette and the target microwell. In the process, we built a system of the resistance-based voltage feedback, with which we could judge the whether the cell was aspired or placed. The target of the paper was to realize the automated operation of the cell in the microwell array that contains the aspiration, transfer and placement with high efficiency.
基金
The Authors thank the support from the National Natural Science Foundation of China （Project Codes： 51005230） and the Education Department of Liaoning province science and technology research projects （Project Codes： L2015452）.
