大鼠弥漫性轴索损伤后皮层和脑干Homer1a蛋白的变化及意义

Expression and significance of Homer1a after brain diffuse axonal injury in rat pallium and brainstem

目的研究侧向旋转致大鼠脑弥漫性轴索损伤后皮层和脑干Homer1a蛋白表达规律及其意义。方法 SD大鼠110只,体重(200±20)g,分为对照组和弥漫性轴索损伤(DAI)组,DAI组应用侧向旋转DAI致伤模型,对照组不予以旋转致伤,其余处理同DAI组,分别在伤后10 min,30 min,1 h,6 h,12 h,24 h,48 h,72 h取大鼠皮层和脑干组织进行Homer1a蛋白分子的免疫组织化学和免疫印迹分析检测。结果两种检测方法发现皮层Homer1a蛋白自伤后30 min起表达,脑干Homer1a自伤后10 min就开始表达,均持续至72 h,皮层各时间点Homer1a表达均弱于脑干组织,而对照组未见该蛋白阳性表达。结论大鼠脑弥漫性轴索损伤后Homer1a蛋白的表达在皮层和脑干组织表达程度有显著性差异,提示DAI后主要以脑干损伤较为严重,Homer1a蛋白分子参与了神经元损伤后脑保护过程。

Objective To study the expression of Homerla in brain diffuse axonal injury in rat pallium and brainstcm. Methods Ninety SD rats, with weight of （200±20） g, were randomized to normal and DAI group. The rat DAI models were produced in DAI group and the normal group was treated in the same way given to the DAI group except for ＂free rotating＂ attack. The rats＇pallium and brainsteam were detected for Homerla by immunoblotting and immunohistochemistry after injury at 10 min, 30 min, 1 h, 6 h, 12 h, 24 h, 48 h, 72 h respectively. Results The Homerla was positive in the pallium after injury from 30 min to 72 h. The Homerla was positive in the brainstem after injury from 10 rain to 72 h. The expression of Homerla in pallium was weaker than in brainstem at every time we detected. Homerla was no expression in normal group. Conclusion The expression of Homerla in pallium and in brainsteam was significant difference, indicates that the pathognomonic of DAI was brain stem inury. The Homerla maybe involved in the brain protection after neuronal injury.