白藜芦醇对低氧内皮细胞活性的影响及机制研究

The research on the effect and mechanism of resveratrol on the inflammatory response of endothelial cells induced by hypoxia

目的探讨白藜芦醇(RSV)对低氧内皮细胞炎症反应的影响及机制。方法 HUVECs分为常氧、低氧、低氧+DMSO、低氧+RSV(低、中、高剂量)组。分别采用CCK8法检测细胞活力,qRT-PCR及Western blot法检测SIRT1、IL-6和ICAM-1 mRNA及SIRT1、PGC-1α和NF-κB p65蛋白的表达,流式细胞术检测活性氧(ROS)含量。结果低氧能抑制HUVECs细胞活力,下调SIRT1、PGC-1α蛋白表达(P<0.05);上调IL-6、ICAM-1 mRNA及磷酸化NF-κB p65蛋白表达(P<0.05);促进ROS生成(P<0.05)。RSV能促进低氧HUVECs细胞活力,上调SIRT1、PGC-1α表达(P<0.05);下调IL-6、ICAM-1及磷酸化NF-κB p65蛋白的表达(P<0.05);抑制ROS生成(P<0.05)。结论 RSV可促进低氧HUVECs细胞活力,其机制可能与RSV抑制低氧HUVECs炎症反应有关。

Objective To study the effect and mechanism of resveratrol on the inflammatory response of endothelial cells induced by hypoxia. Methods Human umbilical vein endothelial cells were divided into normal group,hypoxia group,DMSO group and low,medium and high doses of RSV groups. The cell viability was detected by CCK8 assay,The mRNA contents of SIRT1,IL-6 and ICAM- 1 were detected by real- time PCR method,the protein contents of SIRT1,PGC- 1α and NF- κB p65 were detected by western blot method,the contents of ROS were detected by flow cytometry method. Results Compared with normal group,the cell viability and contents of SIRT1 and PGC- 1α protein were decreased markedly in hypoxia group,the contents of IL- 6 mRNA,ICAM- 1 mRNA,p- NF- κB p65 protein and ROS were increased markedly. Compared with DMOS group,the cell viability and contents of SIRT1 and PGC- 1α protein were increased markedly in RSV groups,the contents of IL- 6 mRNA,ICAM- 1 mRNA,p- NF- κB p65 protein and ROS were decreased markedly in RSV groups. Conclusion RSV could promote the cell viability of HUVECs induced by hypoxia. This maybe be concerned with that RSV could inhibit the inflammatory response of HUVECs induced by hypoxia..