麻黄对大鼠额叶皮层氧化损伤的影响

Ma Huang( Ephedra) induces oxidative stress in the rat prefrontal cortex

目的考察不同剂量麻黄对大鼠额叶皮层部位造成的氧化损伤。方法分别灌胃(ig)给予大鼠生理盐水、麻黄碱(48 mg·kg^(-1))和不同剂量麻黄(30,15,7.5 g·kg^(-1))供试药,连续ig7天。末次灌胃给药后将大鼠断头处死,在冰台上立即取脑,分离额叶皮层部位,测定MDA、NO含量和SOD、CAT、GSH-Px活力。结果与生理盐水组相比,麻黄碱组大鼠额叶皮层部位MDA和NO的含量显著增加,SOD、CAT和GSH-Px活力显著降低;麻黄低剂量组(7.5 g·kg^(-1))大鼠额叶皮层部位MDA的含量明显增加,NO的含量显著增加,SOD、CAT和GSH-Px活力显著降低;麻黄中、高剂量组(15、30g·kg^(-1))大鼠额叶皮层部位MDA、NO的含量显著增加,SOD、CAT、GSH-Px活力显著降低。结论麻黄可以引起大鼠额叶皮层部位氧化损伤,可能是麻黄对大鼠中枢神经系统毒副作用的部分机制。

Objective To study the oxidative stress in the rat prefrontal cortex after administered Ma Huang. Methods On 7 consecutive days,saline,ephedrine and Ma Huang decoction were administered daily. On the 7th day,concentrations of malondialdehyde（ MDA）,nitric oxide（ NO） in the rat prefrontal cortex were quantified. Changes in the activities of superoxide dismutase（ SOD）,glutathione peroxidase（ GSH- Px） and catalase（ CAT） in the rat prefrontal cortex were also determined. Results Oxidative damage in prefrontal cortex of rats as indicated by a rise in MDA and NO concentration and depleted reduced SOD,CAT and GSH- Px activity compared to control group were observed. Compared with the ephedrine group,concentrations of MDA and NO in 7. 5 g·kg^（-1）dose group were lower while 15 and 30 g·kg^（-1）dose groups were higher（ P 〈0. 01）. GSH- Px activity reduced in all the ephedra groups compared to the ephedrine group（ P〈 0. 01）. SOD and CAT activity reduced in 15 and 30 g·kg^（-1）dose group compared to the ephedrine group（ P 〈0. 01）. Conclusion Ma Huang induces oxidative stress in the rat prefrontal cortex,suggesting that these effects are part of the underlying neurotoxic mechanism of Ma Huang in the central nervous system.