Robinsoniella peoriensis携带者检出1例报告分析

One Carrier of Robinsoniella Peoriensis in China

目的了解Robinsoniella peoriensis(R.peoriensis)临床分离菌株的形态特点、生化特征及耐药情况,为深入研究该菌提供资料。方法在对四川大学华西医院ICU医疗单元患者进行肛拭子筛查时,从一例36岁的男性肺癌患者身上分离到了一株R.peoriensis,采用VitekⅡ全自动微生物鉴定及药敏分析系统(BioMérieux,Marcy-I′toile,France)的厌氧菌鉴定卡进行生化鉴定,琼脂稀释法体外药敏试验了解该菌耐药情况。结果生化鉴定示R.peoriensis对蔗糖、β-半乳糖吡喃苷酶吲哚酚、阿拉伯糖苷酶等反应阳性,而对鉴定卡的其他成分反应阴性。体外药敏试验证实该菌对克林霉素、利福平、莫西沙星耐药,而对万古霉素、甲硝唑、四环素敏感。结论 R.peoriensis是一种革兰阳性的厌氧芽孢杆菌,对蔗糖等多种物质反应阳性,既可导致感染又可引起定植。本研究中的R.peoriensis临床分离菌株对克林霉素、利福平、莫西沙星耐药,并且克林霉素和利福平最低抑菌浓度均大于512μg/mL。

Objective To study morphological feature,biochemical characteristic and antibiotic resistance of Robinsoniella peoriensis（R.peoriensis）strain.Methods The clinical R.peoriensis strain was isolated from ananus swab samples being screened in ICU.Biochemical characteristic of the strain was completed by fully automatic microbial identification and drug susceptibility analysis system（BioMérieux,Marcy-l′toile,France）with the ANC VitekⅡ.Antibiotic susceptibility in vitro was performed using agar dilution.Results The organism was found to be positive to saccharose,beta-galactopyr anosidase indoxyl,alpha-arabinosidase and so on,while negative to the others.The susceptibility test in vitro showed that this strain was resistant to clindamycin,rifampicin,moxifloxacin,while sensitive to vancomycin,metronidazole and tetracycline.Conclusion R.peoriensis is positive to many biochemical products such as saccharose.The clinical isolate of R.peoriensis strain is resistant to clindamycin,rifampicin and moxifloxacin.