HPLC法测定枸杞子配方颗粒中甜菜碱的含量

Determination of content of betaine in Wolfberry fruit formula granule by HPLC

目的：建立枸杞子配方颗粒中甜菜碱的含量测定方法。方法：采用高效液相色谱法,乙腈-水（85∶15）为流动相,流速为1.0 ml/min,柱温为30℃,检测波长为200 nm,色谱柱为Kromasil NH2（250 mm×4.6 mm,5μm）。结果：6个厂家13批次的枸杞子配方颗粒中甜菜碱含量介于0.542 8～2.055 6 mg/g之间。结论：该方法操作简便,结果稳定可靠,可用于枸杞子配方颗粒的质量评价。

Objective： To establish an HPLC method for determination of betaine in wolfberry fruit formula granule. Methods：HPLC was performed on a Kromasil NH2（ 250 mm × 4. 6 mm,5 μm） column at 30 ℃ with mobile phase consisting of acetonitrile-water（ 85∶ 15）,the flow rate was 1. 0 ml / min,the detection wavelength was 200 nm. Results： The content of betaine in wolfberry fruit formula granule in 13 batches from six factories ranged between 0. 542 8 ~ 2. 055 6 mg / g. Conclusion： The method is simple and reliable,and can be used for the quality evaluation of betaine in wolfberry fruit formula granule.