病毒感染及相关免疫因子与鼻内翻性乳头状瘤发病关系的研究

Impact of virus infection and related immunity factors on nasal inverted papilloma

目的:探讨人乳头状瘤病毒(HPV)感染及相关免疫因子TGF-β、IL-10、IFN-γ与鼻内翻性乳头状瘤(NIP)及鼻鳞状细胞癌(NSCC)的关系。方法:收集30例NIP(其中7例为复发性NIP)、23例NSCC、19例鼻息肉(NP)组织,采用原位杂交法检测HPV6/11、HPV16/18型的感染情况,采用免疫组织化学技术EnVision二步法检测TGF-β、IL-10、IFN-γ的表达情况。结果:30例NIP、23例NSCC的HPV感染阳性率分别为43.33%、52.17%,19例NP中无一例有HPV感染。23例非复发性NIP中有9例HPV感染,7例复发性NIP中有3例HPV感染。NIP与NP、NSCC与NP之间HPV感染率差异有统计学意义(P<0.05),NIP与NSCC、非复发性NIP与复发性NIP之间HPV感染率差异无统计学意义(P>0.05)。TGF-β、IL-10、IFN-γ因子在NIP、NSCC及NP组中均有表达。NIP、NSCC、NP组间TGF-β免疫组织化学染色程度存在差异(P<0.01),NIP组比NP组、NSCC组间TGF-β的表达增加,NSCC组与NP组间TGF-β的表达无差异。NIP、NSCC、NP组间IL-10的表达存在差异(P<0.01),NIP组比NP组IL-10的表达减少,NIP组与NSCC组间IL-10的表达程度无差异,NSCC组与NP组IL-10的表达程度无差异。NIP、NSCC、NP组间IFN-γ的表达程度无差异(P>0.05)。NIP、NSCC组HPV感染与TGF-β、IL-10、IFN-γ的表达之间无差异(P>0.05)。结论:HPV感染与部分NIP、NSCC的发生有关;HPV感染与NIP恶变为癌的关系尚不明确;HPV感染与NIP复发无关;TGF-β及IL-10的异常表达可能与NIP的发病有关;IFN-γ与NIP的发生、发展无关;HPV感染与NIP、NSCC中TGF-β、IL-10、IFN-γ的表达不相关。

Objective：To explore the relationship between human papilloma virus infection,TGF-β,IL-10,IFN-γand nasal inverted papilloma.To explore the relat-ionship between human papilloma virus infection,TGF-β,IL-10,IFN-γand nasal squamous cell carcinoma.Method：Thirty cases of NIP（including 7cases of recurrent NIP）,23 cases of NSCC,19 cases of NP were collected.In situ hybridization（ISH）was used to detect the infection of HPV6/11,HPV16/18.Immunohistochemical technique（EnVision）was used to detect the expression of TGF-β,IL-10,IFN-γ.Result：The HPV infection rates of 30 cases of NIP,23 cases of NSCC were 43.33%,52.17%.None of the NP cases was infected with HPV.Nine out of 23 cases of primary NIP were positive in HPV infection（39.13%）.Three out of 7cases of recurrent NIP were positive in HPV infection（42.86%）.There were significant differences in the HPV infection rate between NIP and NP as well as between NSCC and NP（P〈0.05）.No significant difference of HPV infection rate was observed between NIP and NSCC or between recurrent NIP and primary NIP（P〉0.05）.TGF-β,IL-10,IFN-γwere expressed in NSCC,NIP and NP.The expression of TGF-βwas significantly different among NIP,NSCC and NP（P〈0.01）.The expression of TGF-βin NIP group was higher than that in NP and NSCC group.There was no significant difference of TGF-βexpression between NSCC and NP group.The expression of IL-10 was significantly different among NIP,NSCC and NP（P〈0.01）.The expression of IL-10 in NIP group was lower than that in NP group.There was no significant difference in IL-10 expression between NIP and NSCC or between NSCC and NP.No significant differences of IFN-γexpression was observed among NIP,NSCC and NP（P〉0.05）.There was no correlation between the expression of TGF-β,IL-10,IFN-γand HPV infection in NIP and NSCC（P〉0.05）.Conclusion：HPV infection is related to the pathogenesis of some NIP and NSCC cases.The relationship of between HPV infection and malignance of NIP is not clear.HPV infection is not related to the recurrence of NIP.Abnormal expression TGF-βand IL-10 may be involved in the pathogenesis of NIP.There is no relationship between IFN-γand the pathogenesis and development of NIP.HPV infection is not related with the expression of TGF-β,IL-10,IFN-γin NIP and NSCC.