摘要
In order to promote marker-assisted selection of Tan sheep, the polymorphism of OB gene in zan sheep was detected by sequencing and PCR-RFLP tech- niques. The correlation between polymorphism of OB gene and weight of Tan sheep was analyzed. The results indicated that there was a CT point mutation at locus 253 in exon 3 region of OB gene, but no mutant homozygote was found. The results of chi-square test showed that the frequency of BB and AB genotypes was con- sistent with Hardy-Weinberg equilibrium. AB genotype was the dominant genotype controlling weight of Tan sheep with the genotype frequency of 0.05. According to the results of correlation analysis, the mutation exerted significant effects on weight of Tan sheep at 3-month-old and 6-month-old （P 〈 0.05）. Moreover, interac- tions between ~enotvoe and ~ender had no sianificant effect on weight of Tan sheen （ P 〉 0.05 ）.
In order to promote marker-assisted selection of Tan sheep, the polymorphism of OB gene in zan sheep was detected by sequencing and PCR-RFLP tech- niques. The correlation between polymorphism of OB gene and weight of Tan sheep was analyzed. The results indicated that there was a CT point mutation at locus 253 in exon 3 region of OB gene, but no mutant homozygote was found. The results of chi-square test showed that the frequency of BB and AB genotypes was con- sistent with Hardy-Weinberg equilibrium. AB genotype was the dominant genotype controlling weight of Tan sheep with the genotype frequency of 0.05. According to the results of correlation analysis, the mutation exerted significant effects on weight of Tan sheep at 3-month-old and 6-month-old （P 〈 0.05）. Moreover, interac- tions between ~enotvoe and ~ender had no sianificant effect on weight of Tan sheen （ P 〉 0.05 ）.
基金
Supported by Special Project of Dominant Characteristic Agriculture Breeding in Ningxia Hui Autonomous Region
Major Project of Agriculture in Ningxia Hui Autonomous Region(2012ZDN1001)
