氟碳乳剂的制备及其对机体供氧能力研究

The Preparation of Perfluorocarbon Emulsion and Its Ability of Oxygen Supply to Organism

探究了以人工肺表面活性物质(APS)为载体的氟碳乳剂对肺癌细胞(A549)生长的影响以及通过肺部给药后对海水淹溺动物肺部氧合能力的改善情况。制备得到了分散性较好的氟碳乳剂,其平均粒径为46.62 nm。通过核磁共振及离子色谱证明了氟碳成功地包封其中,氟碳的含量为23.54%。通过A549细胞共孵育实验,证明了氟碳乳剂能在1%氧浓度下较明显地促进A549细胞增殖;当氟碳乳剂浓度为5 mg/m L时,细胞增殖率达124%,而在正常氧浓度下,其对A549细胞增殖无明显作用。实验兔的海水淹溺模型实验证明了,海水淹溺实验兔氟碳乳剂肺部给药后血氧分压迅速恢复,氟碳乳剂具有一定改善动物肺部氧合的能力。氟碳乳剂在低氧下能促进A549细胞增殖,通过肺部给药后对海水淹溺动物肺部氧合能力有一定的改善,这说明其在通过肺部给药来改善肺部通气应用中有潜在的应用价值。

Artificial pulmonary surfactant （APS） /Perfluorocarbon （PFC） emulsion using APS as a carrier is prepared through high-speed emulsification, the effect of APS/PFC emulsion on cell proliferation and its ability of improving pulmo- nary oxygenation to seawater drowning rabbits are explored. PFC content of APS/PFOB freeze-dried powder is 23.54%. A549 cell viability is increased gradually with the increasing concentration of APS/PFOB emulsion under condition of 1% oxygen concentration. About 124% cell proliferation rate is maintained when the concentration is 5 mg/mL. Moreover, it is demonstrated that APS/PFOB emulsion can improve pulmonary oxygenation to seawater drowning rabbits through pulmonary delivery. APS/PFC emulsion has abilities of promoting A549 cell proliferation during hypoxia and improving pulmonary oxygenation to seawater drowning rabbits through pulmonary delivery, it has potential application for improving lung ventilation.