骨肉瘤患者细胞侵袭相关IL-6、IL-12血清水平检测的临床意义

Clinical Significance of Detection of Cell Invasion Related IL-6 and IL-12 Serum Levels in Osteosarcoma Patients

目的：研究血清IL-6及IL-12水平在骨肉瘤患者与健康体检者之间的差异及临床意义。方法：采用酶联免疫吸附法（ELISA）检测160例骨肉瘤患者及250例健康体检者的外周血血清IL-6及IL-12水平，比较两组间IL-6、IL-12水平的差异及其相关性，探讨血清IL-6及IL-12水平与骨肉瘤发病及进展的关系。结果：骨肉瘤组患者血清IL-6水平明显高于对照组（P〈0.01），IL-12水平明显低于对照组（P〈0.01）。在骨肉瘤患者中，处于肿瘤发病Ⅲ～Ⅳ期的患者其血清IL-12水平低于Ⅰ～Ⅱ期患者（P〈0.05）；合并远处转移的患者血清IL-12水平低于不发生远处转移的患者（P〈0.05）。血清IL-6水平与IL-12水平无明显线性相关（P〉0.05）。结论：血清IL-6及IL-12水平在骨肉瘤患者表达异常，两者在骨肉瘤的发生中可能起免疫调节作用，IL-12的低表达可能与骨肉瘤的进展相关。

Objective：To explore the difference of serum IL-6 and IL-12 levels between the patients with osteosarcoma and healthy individuals,as well as its clinical signifcance.Method：By ELISA,the concentration of serum IL-6 and IL-12 levels were measured in 160 patients with osteosarcoma（osteosarcoma group） and 250 healthy individuals （controls group）.Differences between serum IL-6 and IL-12 levels were compared and analyzed its correlation.The relationship between serum IL-6 and IL-12 levels and the incidence and progression of osteosarcoma were investigated.Result：The levels of serum IL-6 was significantly higher in the osteosarcoma group than in the control group（P〈0.01）,the level of serum IL-12 was significantly lower in the case group than in the controls group （P〈0.01）.Serum IL-12 levels in stages Ⅲ-Ⅳ were lower than those in stages Ⅰ-Ⅱ of osteosarcoma （P〈0.05）.Patients with metastasis had higher IL-12 levels than those without metastasis （P〈0.05）. No associations were found between IL-6 and IL-12 levels（P〉0.05）.Conclusion：This study indicates that serum IL-6 and IL-12 levels abnormal expression in osteosarcoma patients. IL-6 and IL-12 may play the role of immunoregulation in the pathogenesis of osteosarcoma,the lower serum IL-12 levels may be associated with development and progression of osteosarcoma.