摘要
以实验室自制的大麦醇溶蛋白(粗蛋白含量92.6%),通过液-液分散法自组装,制备负载阿魏酸的纳米粒子,当阿魏酸:大麦醇溶蛋白=1∶5(w∶w)时,制备得到的复合纳米粒子粒径为446.2±12.6nm,zeta电位为16.65±0.20mV,稳定性较好;阿魏酸的包埋率为28.35%,载药率为5.37%。对纳米粒子进行扫描电镜(SEM)观察,可见大麦醇溶蛋白纳米粒子分散性良好,呈现均匀规则的圆球状;阿魏酸-大麦醇溶蛋白复合纳米粒子粒径较小,颗粒之间略有黏连。傅里叶变换红外光谱(FTIR)测试表明,大麦醇溶蛋白分子中氢键对应的3322cm^(-1)处吸收峰,在阿魏酸-大麦醇溶蛋白复合纳米粒子中红移至3256cm^(-1),表明二者之间形成了氢键;此外,大麦醇溶蛋白酰胺基I区域和酰胺基II区域的位置分别由1548cm^(-1)和1664cm^(-1)红移至1496cm^(-1)和1636cm^(-1),表明二者之间可能存在静电相互作用。
Hordeinwas extracted from barley （crude protein%=92.6%）. Hordein nanoparticles and ferulic acid- hordein nanoparticles were prepared through liquid-liquid dispersion method and were self-assembled in 55% iso- propanol. Whenferulic acid： hordein （w：w）=l：5, the size ofnano-particles was 446.2＋12.6nm and the zeta potential was 16.65＋0.20mV, which implied great stability; also encapsulation efficiency was 28.35% and drug loading was 5.37%. Scanning electron microscope （SEM） observation showed good dispersion of an almost sepherical morphology for uniformly shaped hordein nanoparticles; ferulic acid-hordein nanoparticles were smaller in particle size and were slightly adhesive with each other. The FTIR results showed that the hydrogen bands of hordeinmolecules was at 3322cm-l andred shifted to 3256cm-1 thatof ferulic acid-hordeinnanoparticles which may imply the formation of hy- drogen bonds between ferulic acid and hordein.Moreover, hordein amide I region and amide II region were red shifted from 1548cm-I and 1664cm-1 to 1496cm-1 and 1636cm-1, which showed cross-linking betweenferulic acid and hordein through electrostatic interactions.
出处
《食品与发酵科技》
CAS
2016年第3期11-14,共4页
Food and Fermentation Science & Technology
