不同静压力对新生SD大鼠髁突软骨细胞增殖与凋亡的影响

Effects of the static compressive stress on the proliferation and apoptosis of condylar chondrocytes in vitro

目的:就不同静压力对新生SD大鼠髁突软骨细胞增殖与凋亡的影响进行探讨。方法:加载压力分别为36k Pa、24k Pa、12k Pa、0k Pa,持续静压1h,在加力后立即(0h)收集细胞进行检测,并且开展流式细胞术检测。结果:当加载压力分别为36k Pa、12k Pa时,与0k Pa相比,髁突软骨细胞的各个增殖、凋亡指数都呈现出明显的下降趋势,具有统计学意义(P<0.05)。当加载压力为12k Pa时,细胞凋亡指数减幅最大;当加载压力为36k Pa时,细胞增殖指数减幅最大。结论:不同静压力对新生SD大鼠髁突软骨细胞增殖与凋亡有一定的影响,但并非线性关系,值得深入研究。

Objective： To discuss the effects of different static compressive stress on proliferation and apoptosis of MCC of neonatal SD rats. Methods： Loading stress was 36 k Pa、24 k Pa、12 k Pa、0 k Pa,respectively,and static pressure for 1h. The cells were examined immediately（ 0h） after stress application,and detected by using flow cytometry. Results： When the stress was 36 k Pa,12 k Pa respectively,compared with 0k Pa,its proliferation and apoptosis index of MCC declined sharply,which showed statistically significant differences（ P〈0. 05）. When the stress was 12 k Pa,the damping of apoptosis was the biggest. When the stress was 36 k Pa,the damping of proliferation was the biggest. Conclusion： Different static compressive stress have some performance impacts on proliferation and apoptosis of MCC of neonatal SD rats,but it is nonlinear relationship,which needs intensive study.