柠檬酸钠对小鼠生理及肠道菌群的影响

Effect of sodium citrate on physiological properties and composition of intestinal microbiota of mice

研究添加剂柠檬酸钠对小鼠的生长、血清生化指标及肠道菌群的影响。小鼠随机分为空白组、低剂量组(添加量0.65%)、中剂量组(添加量2.6%)和高剂量组(添加量5.2%),分别连续膳食干预4周,于不同时间点采集各组小鼠粪便样品并记录体重、采食量等数据;试验结束后对小鼠血清生化指标和肠道菌群进行分析,结果表明:柠檬酸钠组小鼠体重均低于空白组,且高剂量组小鼠的血清高密度脂蛋白胆固醇含量显著增高(P<0.05)。柠檬酸钠的添加影响了小鼠肠道微生物的beta多样性,使厚壁菌门和拟杆菌门的比例发生变化。在属水平上,柠檬酸钠高剂量组检测出了棒状杆菌属和葡萄球菌属,说明摄食高剂量的柠檬酸钠会对小鼠肠道微生态造成一定影响。

Effects of sodium citrate were investigated on the growth,the serum biochemical parameters and intestinal flora of mice.Mice were randomly divided into four groups：control group,low-dose group（added 0.65% sodium citrate）,middle-dose group（added2.6% sodium citrate）and high-dose group（added 5.2% sodium citrate）.The diet intervention lasted for four weeks.Stool samples and weight data were collected weekly,and the composition of intestinal microbiota and the serum biochemical parameters were determined at the end of the fourth week.Results showed that the addition of sodium citrate made the body weight of mice less than control group.And the high-dose group＇s high density lipoprotein cholesterol（HDLC）had a significantly increased.The beta diversity and the ratio of Fimicutes to Bacteriodetes were changed by intake of sodium citrate.At the genus level,Corynebacterium and Staphylococcus were detected in the high-dose group.Results indicated that the intestinal flora of mice could be affected by intake of high dose of sodium citrate.