长时间跑台运动前后及恢复期男性大学生外周血CD14+单核细胞TLR2和TLR4表达的变化

The Influence of Prolonged Treadmill Running on CD14^+ Monocyte Toll-like Receptor 2 and 4 Before and After Exercise in Male College Students

目的:研究长时间跑台运动前后及恢复期男性大学生外周血CD14^+单核细胞TLR2和TLR4表达的变化规律。方法:采集12名田径专项男性大学生1.5h跑台运动前后及恢复期外周血,采用流式细胞术检测CD14^+单核细胞表面TLR2和TLR4的表达。结果:经过长时间运动后TLR2表达呈下降趋势,但在运动后即刻和恢复期均未呈现出显著性差异(p>0.05)。运动后即刻和恢复期TLR4表达呈现下降趋势,与运动前相比均呈显著性差异(p<0.05)。结论:长时间运动可导致CD14^+单核细胞表面TLR4表达显著性下降,这可能与长时间运动后的免疫抑制有关。

[Objective]To study the variation of CD14＋ monocyte Toll-like receptor 2 and 4 before and after prolonged treadmill running.[Methods]12 endurance trained male college students had blood samples taken before and after 1.5 h of running, and the expression of TLR2 and TLR4 on CD 14＋ monocyte were analysed using flow ctyometry. [Results] After running, the expression of TLR2 slightly decreased after running（p〉0.05）,the expression of TLR4 decreased significantly（p〈0.05）.[Conelusion] Prolonged running reduces TLR4 significantly, which may be associated with post-exercise immunodepression.